水稻不同生育期根际溶磷菌群落特征OA北大核心

To assess the effects of rhizosphere soil bacteria on phosphorus availability at various rice growth stages,we studied four varieties:Huanghuazhan,Guiyefeng,Zhongkezaofajing No.1,and Sasanishiki.Soil samples from the rhizosphere were gathered at the flowering and maturity phases.We used high-throughput sequencing to examine the rhizosphere phosphate-solubilizing bacterial community,particularly the phoD gene.The study also investigated correlations with soil physicochemical characteristics and phosphorus fractions.The results showed that Pseudomonadaceae(23.52%±31.03%)and Nitrobacteraceae(11.69%±5.07%)dominated the rice rhizosphere as phosphorus solubilizing bacteria.Bacterial diversity at maturity,with Chao1 index of 105.03±9.75 and Shannon index of 1.79±0.13,was significantly greater compared to that flowering stage,which had Chao1 index of 99.23±19.43 and Shannon index of 1.50±0.55.Soil pH,available phosphorus,and total nitrogen were key factors affecting the phosphorus solubilizing bacterial community structure.Significant correlations between the relative abundances of Halomonadaceae,Phyllobacteriaceae,and Burkholderiaceae with different phosphorus fractions underscored their roles in the rice rhizosphere phosphorus cycle.