猕猴桃几丁质酶基因家族的鉴定和表达特征分析
Identification and Expression Characteristics of Chitinase Gene Families in Kiwifruit
摘要
Abstract
In order to explore the characteristics and functions of the chitinase gene family(CHis),eighteen AcCHi genes were identified from Actinidia chinensis.The phylogenetic analysis showed that they clustered in GH18 and GH19 clusters and were divided into 6 groups with high similarity in conserved domain,gene structure and protein structure among the members.The cis-acting elements of AcCHi genes promoter region were mainly involved in hormone response,abiotic stress response and growth and development regulation.The 18 AcCHi genes were unevenly distributed on 12 chromosomes,and 5 of them had fragment duplication.The expression of AcCHis might be regulated by miR172 and miR408.In AcCHis,the transcription of GH18 members and GH19 members in different tissues,such as mature fruit,young fruit,leaf,stem,flower,was significantly different.During the storage of kiwifruit fruit,90.9%of GH18 members had no or low expression,however,71.4%of GH19 members showed differential expression.The expression of AcCHi8 in fruits was up-regulated at 25℃for 1 week and at 4℃for 1 to 3 weeks,and the expression of AcCHi8 was the highest at 4℃for 3 weeks(188 times to the control),while the expression of AcCHi8 was down-regulated at 25℃for 1 week after ABA soaking.Therefore,it was suggested that AcCHi genes might be involved in the process of storage and softening of postharvest fruits.关键词
几丁质酶/猕猴桃/基因鉴定/基因表达Key words
Chitinase/Actinidia chinensis/Gene identification/Gene expression引用本文复制引用
刘晓驰,路喻丹,冯新,高敏霞,赖瑞联,陈桂信,陈义挺..猕猴桃几丁质酶基因家族的鉴定和表达特征分析[J].热带亚热带植物学报,2025,33(1):1-14,14.基金项目
福建省属公益类科研院所基本科研专项(2023R1087) （2023R1087）
福建省人民政府与中国农业科学院"5511"协同创新工程项目(KXXYJBG2021006) （KXXYJBG2021006）
福建省农业科学院科技创新团队建设项目(CXTD2021009-2) （CXTD2021009-2）
"十四五"福建省种业创新与产业化工程项目(zycxny2021010) （zycxny2021010）
中央引导地方科技发展专项(2023L3025)资助This work was supported by the Project for Basic Research of Public Welfare Research Institute in Fujian(Grant No.2023R1087),the Project for"5511"Collaborative Innovation from the People's Government of Fujian Province&Chinese Academy of Agricultural Sciences(Grant No.KXXYJBG2021006),the Project for Science and Technology Innovation Team Building in Fujian Academy of Agricultural Sciences(Grant No.CXTD2021009-2),the Project for Seed Industry Innovation and Industrialization in Fujian(Grant No.zycxny2021010),and the Project of Central Government Guide Local Science and Technology Development(Grant No.2023L3025). （2023L3025）
