热带亚热带植物学报2025，Vol.33Issue(1)：15-24,10.DOI:10.11926/jtsb.4851
三叶木通叶片解剖结构和生理特征对酸雨胁迫的响应和钛的缓解效应
Response of Anatomical Structure and Physiological Characteristics of Akebia trifoliata Leaves to Acid Rain Stress and Relieving Effect of Titanium
摘要
Abstract
To explore the response mechanism of Akebia trifoliata to acid rain stress and the effect of exogenous titanium[Ti(SO4)2]on its acid resistance,the changes in leaf anatomy and physiological characteristics were studied after simulated acid rain and exogenous titanium treatment,and the principal component analysis and correlation analysis were performed.The results showed that compared with the control,the lateral vein and epidermal structure of leaves were seriously damaged under simulated acid rain(T0)treatment,the main vein,main vein vascular bundle,leaf and palisade tissues were thickening,the chlorophyll content was significantly decreased,the oxygen free radical production rate,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)were significantly increased.Exogenous titanium treatments(T1-T3)effectively alleviated the damage of lateral vein and epidermal of leaves,significantly increased chlorophyll content,SOD,POD and CAT activities,and maintained a low rate of oxygen free radical production.Principal component analysis showed that main vein,vascular bundle and leaf thickness played a key role in response mechanism to acid rain stress.Correlation analysis showed that there were significantly positive correlations among vascular bundle thickness,palisade tissue thickness,leaf tissue structure tightness,activities of SOD,POD and CAT.Therefore,the seedlings of A.trifoliata could adapt to acid rain stress by changing the leaf anatomical structure and increasing the activities of SOD,POD and CAT.Appropriate Ti could effectively alleviate the damage caused by acid rain stress,and 0.2 mmol/L Ti had the best effect.关键词
三叶木通/酸雨胁迫/解剖结构/生理特征/耐酸性/钛Key words
Akebia trifoliata/Acid rain stress/Anatomic structure/Physiological characteristics/Acid resistance/Titanium引用本文复制引用
王凯,陶兴梅,李小琴,谯祖勤,刘朝,张永福..三叶木通叶片解剖结构和生理特征对酸雨胁迫的响应和钛的缓解效应[J].热带亚热带植物学报,2025,33(1):15-24,10.基金项目
国家自然科学基金项目(32060645) （32060645）
云南省地方本科高校基础研究联合专项(202101BA070001-036,202101BA070001-172) （202101BA070001-036,202101BA070001-172）
云南省科技厅基础研究专项(202301AU070137) （202301AU070137）
云南省教育厅科学研究基金项目(2023Y0876)资助This work was supported by the National Natural Sciences Foundation of China(Grant No.32060645) （2023Y0876）
the Joint Special Project for Basic Research of Local Undergraduate University in Yunnan(Grant No.202101BA070001-036,202101BA070001-172) （Grant No.202101BA070001-036,202101BA070001-172）
the Program for Basic Research of Department of Science and Technology inYunnan(Grant No.202301AU070137) （Grant No.202301AU070137）
and the Program for Basic Research of Department of Education in Yunnan(Grant No.2023Y0876). （Grant No.2023Y0876）
