热带亚热带植物学报2025，Vol.33Issue(1)：42-48,7.DOI:10.11926/jtsb.4858
金线莲ArCRC基因的克隆、亚细胞定位和表达分析
Cloning,Subcellular Localization and Expression Analysis of ArCRC Gene in Anoectochilus roxburghii
摘要
Abstract
To understand the function of CRABS CLAW(CRC)subgene of the YABBY gene family of Anoectochilus roxburghii in flower and leaf development,the ArCRC gene was cloned by RT-PCR based on the full-length transcriptome data of A.roxburghii.The bioinformatics,prokaryotic expression and subcellular localization of encoded ArCRC protein were analyzed,and the expression pattern of ArCRC gene was analyzed by qPCR.The results showed that the CDS length of ArCRC gene was 576 bp(GenBank accession No.:OR394646),coding 191 amino acids.ArCRC contains YABBY superfamily and HMG-box_SF superfamily conserved domain,with molecular weight 21.514 kD,theoretical isoelectric point 9.16,instability coefficient 41.12,belonging to the unstable protein.Phylogenetic analysis showed that ArCRC was clustered with OsDL of rice(Oryza sativa)and AtCRC of Arabidopsis thaliana,belonging to the CRC subfamily and localized in the nucleus.SDS-PAGE electrophoresis and Western blot results showed that ArCRC gene could be successfully induced in Escherichia coli.qRT-PCR analysis showed that the expression of ArCRC gene was the highest in flowers,followed by leaves,and the expression in the midrib of leaves was significantly higher than that in the margin of leaves.Therefore,it was speculated that ArCRC gene mainly played a role in the development of flower organs and was also involved in regulating the development of the midrib of leaves.关键词
金线莲/ArCRC基因/转录因子/基因克隆/亚细胞定位/基因表达分析Key words
Anoectochilus roxburghii/ArCRC gene/Transcription factor/Gene cloning/Subcellular localization/Gene expression analysis引用本文复制引用
林江波,邹晖,黄惠明,李和平,戴艺民..金线莲ArCRC基因的克隆、亚细胞定位和表达分析[J].热带亚热带植物学报,2025,33(1):42-48,7.基金项目
福建省属公益类科研院所基本科研专项(2021R1030001) （2021R1030001）
福建省农业科学院科技创新团队(CXTD2021001-2)资助This work was supported by the Project for Basic Research of Public Research Institute in Fujian(Grant No.2021R1030001),the Project for Science and Technology Innovation Team of Fujian Academy of Agricultural Sciences(Grant No.CXTD2021001-2) （CXTD2021001-2）
