热带亚热带植物学报2025，Vol.33Issue(1)：91-99,9.DOI:10.11926/jtsb.4915
溪黄草提取物对香蕉炭疽菌的抑制活性和抑菌机理
Antifungal Activity and Potential Mechanism of Isodon serra Extract Against Colletotrichum musae
摘要
Abstract
In order to explore the natural antifungal agent of controlling banana anthracnose,antifungal activity of Isodon serra extract against Colletotrichum musae was evaluated by inhibition of mycelia growth and controlling activity test on banana fruits,and the potential mechanisms were investigated by studying the effect of I.serra extract on the mycelia cell integrity,mycelia morphology and activity of enzyme related to energy metabolism.The results showed that ethanol extract of I.serra and its different polar solvent extraction fractions had obvious antifungal activity against C.musae.Among them,petroleum ether fraction had the strongest inhibitory ability,its medium inhibitory concentration on mycelia growth was 0.18 mg/mL.The test of controlling activity on banana anthracnose showed that different concentrations of petroleum ether fraction decreased the lesion diameter of banana significantly compared with the control group(P<0.05),and the lesion diameter of banana treated with the extract at 4 mg/mL had no significant difference with the positive control(P>0.05).The integrity of C.musae mycelia cell was damaged significantly after incubation with the petroleum ether fraction,leading to the increase of cell permeability,moreover,the plasmolysis,shrinkage and crack in mycelia were also observed,and the damage degree was concentration-dependent.The enzyme activities of succinate dehydrogenase,malate dehydrogenase,ATP synthase and ATPase decreased by 23.56%,60.51%,9.01%and 32.01%respectively with the petroleum ether fraction treatment.In conclusion,these findings suggest that the antifungal mechanisms of I.serra extract against C.musae could be attributed to the disruption of cell structure and the effect on enzyme activities related to energy metabolism.Isodon serra extract could be used as a potential fungicide of controlling banana anthracnose.关键词
溪黄草/提取物/香蕉炭疽菌/抑菌活性/抑菌机理Key words
Isodon serra/Extract/Colletotrichum musae/Antifungal activity/Antifungal mechanism引用本文复制引用
吴妙鸿,林宝妹,林巧莉,吴水金,李跃森,张帅,李海明..溪黄草提取物对香蕉炭疽菌的抑制活性和抑菌机理[J].热带亚热带植物学报,2025,33(1):91-99,9.基金项目
福建省属公益类科研院所基本科研专项(2023R1028003) （2023R1028003）
福建省农业科学院自由探索科技创新项目(ZYTS2023006) （ZYTS2023006）
漳州市自然科学基金项目(ZZ2023J10)资助This work was supported by the Special Project for Public Welfare for Research Institute in Fujian(Grant No.2023R1028003),the Project for Free Exploration Science and Technology Innovation of Fujian Academy of Agricultural Sciences(Grant No.ZYTS2023006),and the Project for Natural Science in Zhangzhou(Grant No.ZZ2023J10). （ZZ2023J10）
