上海针灸杂志2025，Vol.44Issue(2)：229-238,10.DOI:10.13460/j.issn.1005-0957.2024.13.3001
电针通过Hippo/YAP通路调控卵巢早衰小鼠卵巢功能
Electroacupuncture regulates ovarian function in mice with premature ovarian failure via Hippo/YAP pathway
摘要
Abstract
Objective To observe the molecular mechanism of electroacupuncture(EA)in improving ovarian function and regulating the Hippo/YAP pathway in mice with premature ovarian failure(POF)induced by high fat and high sugar.Method Forty-five female C57BL/6 mice were randomized into a control group(wild type,WT),a POF group,and an EA group,with 15 mice in each group.After the successful modeling with a high-fat and high-sugar diet,the EA group received EA at Guanyuan(CV4)and bilateral Zusanli(ST36)and Sanyinjiao(SP6)points.hematoxylin-eosin(HE)staining was adopted to observe ovarian pathological changes,Masson staining was used to observe ovarian fibrosis,and filipin staining was employed to detect ovarian total cholesterol in the mice.The enzyme-linked immunosorbent assay(ELISA)was used to determine the levels of serum follicle-stimulating hormone(FSH),anti-Müllerian hormone(AMH),estradiol(E2),triglyceride(TG),total cholesterol(TC),high-density lipoprotein(HDL),and low-density lipoprotein(LDL).Immunofluorescence staining was used to label the expression of follicle-stimulating hormone receptor(FSHR),proliferating cell nuclear antigen(PCNA),and proliferation marker ki67 in the mice's ovarian granulosa cells(GCs).The reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR)was adopted to detect the mRNA expression of Hippo/YAP pathway genes YAP,TEAD1,TAZ,and MST1,proliferation-related gene PCNA,and apoptosis-related genes BAX and BCL2.Western blot was used to measure the expression of Hippo/YAP pathway proteins p-YAP,YAP,TEAD1,TAZ,and MST1 and apoptosis-related proteins BAX and BCL2.Result Compared to the POF group,the EA group had less follicular atresia,more mature follicles,reduced ovarian fibrosis,and a lower ovarian TC level after the intervention.After the intervention,the EA group showed improvement in sex hormone and lipid metabolism levels:the FSH level dropped significantly(P＜0.05),and the AMH and E2 levels increased significantly(P＜0.05);the levels of TG and TC decreased markedly(P＜0.05),the HDL level increased significantly(P＜0.05),and the LDL level dropped markedly(P＜0.05).In the EA group,the expression of FSHR,PCNA,and ki67 was up-regulated after the intervention.EA intervention activated the HIPPO/YAP pathway,promoting the proliferation of GCs and inhibiting the apoptosis of GCs.Conclusion EA may improve ovarian function by regulating the Hippo/YAP way,and EA can promote the proliferation and suppress the apoptosis of GCs via the Hippo/YAP pathway.关键词
电针/卵巢早衰/Hippo/YAP通路/增殖/凋亡/小鼠Key words
Electroacupuncture/Premature ovarian failure/Hippo/YAP pathway/Proliferation/Apoptosis/Mice分类
医药卫生引用本文复制引用
凌乐乐,王聪,胡寅杰,李冰融,刘鹏,张开勇,李斯雯,刘特,张必萌..电针通过Hippo/YAP通路调控卵巢早衰小鼠卵巢功能[J].上海针灸杂志,2025,44(2):229-238,10.基金项目
上海市进一步加快中医药事业发展三年行动计划2021年-2023年[ZY(2021-2023)-0208] （2021-2023）
