世界科学技术-中医药现代化2025，Vol.27Issue(1)：14-23,10.DOI:10.11842/wst.20240725004
外源激素结合变温层积对黄连种子后熟与萌发的影响
Effects of Exogenous Hormones Combined with Thermotropic Stratification on Post-Ripening and Germination of Coptis chinensis Seeds
摘要
Abstract
Objective In this study,exogenous hormones combined with variable temperature stratification were used to relieve seed dormannia of Coptis chinensis and promote seed germination,and the effects of gibberellin on the physiological,biochemical and embryonic morphological changes of C.chinensis seeds during post-mature germination were further evaluated.Methods Using orthogonal test,different exogenous hormones(GA3,6-BA,NAA,ABA)and different temperature changing stratification(20℃/4℃)were set up,and the germination index(germination rate and split rate)of C.chinensis seeds were statistically compared.The activity of antioxidant enzymes(peroxidase POD,catalase CAT),the content of malondialdehyde(MDA)and endogenous hormones(GA,IAA,ABA)during the germination of C.chinensis seeds under optimal treatment were measured.The microscopic structure of the seeds was observed by paraffin embedding section.Results The results of range analysis showed that the best budding method was:100 mg·L-1 of gibberellin for 12 h,20℃for 60 day,4℃for 10 day.Physiological and biochemical index analysis showed that 100 mg·L-1 exogenous gibberellin treatment promoted the endogenous GA content to reach the peak in advance,maintained the POD and CAT contents at a higher level,and maintained the malondialdehyde(MDA)content at a lower level.The transformation from torpedo embryo to cotyledonous embryo was observed by electron microscope during postmature germination.Conclusion Exogenous gibberellin combined with variable temperature stratification treatment could significantly accelerate seed germination of C.chinensis,mainly by promoting seed embryo morphological development,affecting the contents of GA,POD and CAT,and rapidly relieving seed dormancy to promote seed germination.Compared with natural conditions,the germination period could be shortened by about six months.关键词
黄连种子/赤霉素/变温层积/后熟作用/萌发Key words
Coptis chinensis seeds/Gibberellin/Variable temperature stratification/Afterripening/Germinate分类
农业科技引用本文复制引用
汪思雨,李国凤,杨思雨,陈士林,刘迪,刘义飞..外源激素结合变温层积对黄连种子后熟与萌发的影响[J].世界科学技术-中医药现代化,2025,27(1):14-23,10.基金项目
国家自然科学基金委员会青年科学基金项目(32100299):基于重测序的黄连种质资源综合评价及重要产量品质性状的全基因组关联分析,负责人:刘迪 （32100299）
湖北省中央引导地方科技发展专项--中药炮制与饮片等级研究创新服务平台(2022BGE201). （2022BGE201）
