世界科学技术-中医药现代化2025，Vol.27Issue(1)：24-35,12.DOI:10.11842/wst.20241121003
野菊细胞色素P450基因家族全基因组鉴定及黄酮合酶基因分析
Genome-Wide Identification of Cytochrome P450 Gene Family and Analysis of Flavonoid Synthase Genes in Chrysanthemum Indicum
摘要
Abstract
Objective Systematic identification and analysis of the cytochrome P450(CYP450)gene family members and their functions in Chrysanthemum indicum L.to provide a basis for further studies on their roles in the flavonoid synthesis pathway.Methods Based on the genomic data of a diploid Chrysanthemum indicum L.,the members of the P450 gene family were identified and analyzed using bioinformatics methods.Moreover,the transcriptome and quasi-targeted metabolome analysis were combined to screen the flavone synthase Ⅱ(FNSII)genes from the CYP93 family,and their expression levels were tested in different tissues of C.indicum using real-time fluorescence quantitative PCR(qPCR).Results A total of 460 P450 genes were identified from the C.indicum genome,belonging to 43 families within 8 family clans.The encoded proteins ranged from 336 to 1538 amino acids in length,with a relative molecular mass between 38.01 to 175.00 kDa,and an isoelectric point between 5.61 to 9.71.Chromosome localization analysis indicated that these 460 P450 genes were unevenly distributed on nine chromosomes,with the highest number of light-responsive elements in the promoter regions.Expression pattern analysis showed that the expression levels of two FNSII genes(CindChr2G00102820.1 and CindChr8G00552890.1)were significantly higher in leaf of C.indicum than in flower.Conclusion The comprehensive analysis of the P450 gene family in C.indicum is helpful to further elucidate the function of the P450 gene family in flavonoid biosynthesis.关键词
野菊/P450基因家族/FNSII/表达分析Key words
Chrysanthemum indicum/P450 gene family/FNSII/Expression analysis分类
医药卫生引用本文复制引用
赵彬凯,陈诗,廖家豪,雷笛,张景景,刘义飞..野菊细胞色素P450基因家族全基因组鉴定及黄酮合酶基因分析[J].世界科学技术-中医药现代化,2025,27(1):24-35,12.基金项目
国家自然科学基金委员会面上项目(32270231):菊属植物TS/CYP基因簇的起源进化机制及功能研究,负责人:刘义飞. （32270231）
