世界科学技术-中医药现代化2025，Vol.27Issue(1)：65-74,10.DOI:10.11842/wst.20240603005
基于SSR分子标记的少棘巨蜈蚣遗传多样性及遗传结构分析
Genetic Diversity and Genetic Structure Analysis of Scolopendra subspinipes mutilans Based on SSR Markers
摘要
Abstract
Objective Based on the transcriptome data of Scolopendra subspinipes mutilans L.Koch,SSR primers were designed and developed to evaluate the genetic diversity and analyze the population structure of the germplasm resources of S.mutilans,which can provide a theoretical basis for the development and utilization of the germplasm resources of S.mutilans.Methods We collected 301 samples of S.mutilans from major production areas,and analyzed the population genetic diversity and population structure of the germplasm resources of S.mutilans using 18 pairs of highly polymorphic SSR primers after screening.Results A total of 142 alleles were amplified by 18 primer pairs across all samples,with an average of 7.89 alleles amplified per locus.The mean of observed heterozygosity(HO)of the 18 populations was 0.39,the mean of expected heterozygosity(HE)was 0.46,the mean of polymorphism information content(PIC)was 0.41,the mean of Nei's gene diversity index(H)was 0.46,and the mean of Shannon information index(I)was 0.81.The results showed that the wild resources of S.mutilans collected in this study had a high level of genetic diversity,Four populations in Anhui and Zhejiang had higher genetic diversity than populations in Hubei.Molecular analysis of variance(AMOVA)of the population showed that most of the genetic variation in the materials of this study mainly occurred within populations.The STRUCTURE analysis of germplasm resources showed that all germplasm could be divided into four populations.Conclusion This study showed that the natural population of S.mutilans possesses high genetic diversity with high genetic variation and is better adapted to changes in the environment.Our results provided a research basis for the rational utilization of the germplasm resources of S.mutilans and the selection and breeding of superior varieties.关键词
少棘巨蜈蚣/简单重复序列/遗传多样性/种群结构Key words
Scolopendra subspinipes mutilans L.Koch/SSR/Genetic diversity/Population structure分类
医药卫生引用本文复制引用
彭凌风,邓犇,张凯,淦胜坤,汪苗苗,皮强强,刘合刚,胡志刚..基于SSR分子标记的少棘巨蜈蚣遗传多样性及遗传结构分析[J].世界科学技术-中医药现代化,2025,27(1):65-74,10.基金项目
刘合钢全国名老中医药专家传承工作室建设项目,负责人:刘合刚 （）
湖北中医药大学重大项目(1018070111):基于中医临床的蜈蚣产业关键技术研究与应用,负责人:胡志刚. （1018070111）
