塑料科技2025，Vol.53Issue(1)：131-136,6.DOI:10.15925/j.cnki.issn1005-3360.2025.01.024
HPLC-MS/MS法测定塑料类食品接触材料制品中邻、间、对苯二甲酸二(2-乙基)己酯的特定迁移量
Determination of Specific Migration Amounts of DEHP,DOIP and DOTP in Plastic Food Contact Material and Products by HPLC-MS/MS Method
摘要
Abstract
A high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established for the simultaneous determination of the specific migration amounts of di(2-ethylhexyl)phthalate(DEHP),di(2-ethylhexyl)isophthalate(dioctyl isophthalate,DOIP)and di(2-ethylhexyl)terephthalate(dioctyl terephthal,DOTP)in plastic food contact material and products.1.00 mL of the well-mixed 10%ethanol,3%acetic acid,4%acetic acid,20%ethanol and 50%ethanol or isooctane soaking solution sample of plastic food contact material products was accurately pipetted into a 10 mL volumetric flask,the volumetric flask was made up to the mark with isopropanol and mixed well,and then the solution of the volumetric flask was passed through a needle filter membrane into an injection vial for testing.Using methanol and 0.1%formic acid water as mobile phases,the baseline separations of DEHP,DOIP and DOTP were achieved in a C18 column within 16 minutes,and the tandem mass spectrometry was operated in ESI and MRM modes.In the food simulant soaking solutions of 10%ethanol,4%acetic acid,50%ethanol or isooctane of polypropylene(PP)plastic cups,the recovery rates of DEHP,DOIP and DOTP at three spiking levels were ranged from 83.8%to 113.0%,with relative standard deviations(RSDs)from 1.4%to 8.3%.The linearities of DEHP,DOIP and DOTP were good with R≥0.999 4 in the concentration range of 0.010～0.200,0.100～2.000,0.500～10.000 µg/L respectively,and the quantitative limits of DEHP,DOIP and DOTP were 0.020,0.300,1.300 mg/kg respectively.The method is fast,simple and accurate,and the quantitative limits also meet the requirements of standards and regulations.It can effectively avoid the confusing errors in detection results caused by incomplete chromatographic separation of isomers with different limits and similar mass spectrometry confirmation ion pairs.It has been applied in the detection of DEHP,DOIP and DOTP in actual samples.关键词
高效液相色谱-串联质谱法/DEHP/DOIP/DOTP/特定迁移量Key words
HPLC-MS/MS/DEHP/DOIP/DOTP/Specific migration amount分类
化学化工引用本文复制引用
刘萍,周丽群,臧珊珊,金晶,童新,喻雅萍..HPLC-MS/MS法测定塑料类食品接触材料制品中邻、间、对苯二甲酸二(2-乙基)己酯的特定迁移量[J].塑料科技,2025,53(1):131-136,6.基金项目
2023年度高校国内访问工程师"校企合作项目"(FG2023321) （FG2023321）
台州科技职业学院2023年度校级课题(23QNZ01) （23QNZ01）
