双硫死亡相关LncRNA在胃癌中的表达及其临床意义OACSTPCD

Objective To construct prognostic model of disulfidptosis-related LncRNA(DRLncs)and explore the mechanism of LncRNA-small nucleolar RNA host genes(SNHG4)in promoting the progression of gastric cancer.Methods The multi-omics features of disulfidptosis genes in gastric cancer were first analyzed based on The Cancer Genome Atlas(TCGA)database.DRLncs were further screened,and disulfidptosis related gene recurrence score(DRG-RS)model was constructed to predict the overall survival(OS)and correlation with the clinical characteristics of gastric cancer patients.Differential gene analysis,functional enrichment and the correlation between chemotherapy efficacy in high-and low-risk groups were analyzed.Cell-counting kit-8(CCK-8)assay and migration assay confirmed that LncRNA-SNHG4 promoted the malignant phenotype of gastric cancer cells.Western Blotting assay was used to detect the signaling pathway regulated by LncRNA-SNHG4.Results DRG-RS model including 8 DRLncs was successfully constructed,showing good performance in predicting OS of the patients with gastric cancer.DRG-RS model was significantly correlated with tumor-related signaling pathways,clinical characteristics and drug sensitivity of gastric cancer patients.LncRNA-SNHG4 expression was significantly increased in gastric cancer.LncRNA-SNHG4 promoted the proliferation and migration of gastric cancer cells.LncRNA-SNHG4 promoted the activation of Wnt signaling pathway in gastric cancer cells.Conclusions The DRG-RS model constructed in our study can stratify the prognosis of gastric cancer,and is closely related to malignant characteristics and chemotherapy efficacy of gastric cancer patients.The expression of LncRNA-SNHG4 significantly increased in gastric cancer tissues,and LncRNA-SNHG4 promoted the malignant phenotype of gastric cancer cells through the Wnt signaling pathway,suggesting that LncRNA-SNHG4 may play an important role in the progression of gastric cancer.