心脑血管病防治2024，Vol.24Issue(12)：7-10,28,5.DOI:10.3969/j.issn.1009-816x.2024.12.003
微小RNA-20a-5p及E2F转录因子3在心力衰竭患者中的表达情况及临床意义
Expression and clinical significance of microRNA-20a-5p and E2F transcription factor 3 in heart failure patients
摘要
Abstract
Objective To investigate the expression and clinical significance of microRNA-20a-5p(miR-20a-5p)and E2F transcription factor 3(E2F3)in heart failure(HF)patients based on data from the Gene Expression Omnibus(GEO)database.Methods Differentially expressed microRNAs(miRNAs)and their target messenger RNAs were screened in the GSE136547 and GSE104150 datasets.Cytoscape was used to analyze key differentially expressed miRNAs.Eighty HF patients treated at the Affiliated Changshu Hospital of Nantong University from January to December 2020 were selected as the HF group,while 80 individuals with normal left ventricular systolic function who underwent routine health examinations during the same period were selected as the control group.Clinical data were collected and compared between the two groups.Key differentially expressed miRNAs and their target genes were validated using qPCR in both groups.Receiver operating characristic(ROC)curve analysis was conducted to assess the diagnostic value of the key differentially expressed miRNAs and their target genes for HF.Additionally,Pearson correlation analysis was performed to evaluate the correlations between the key differentially expressed miRNAs and their target genes and left ventricular ejection fraction(LVEF)and B-type natriuretic peptide(BNP).Results There were 11 differentially expressed miRNAs in the GSE136547 dataset and 170 in the GSE104150 dataset,with 6 overlapping.Two key differentially expressed miRNAs were identified by Cytoscape analysis:miR-20a-5p and miR-15a-5p,with E2F3,ABL proto-oncogene 2(ABL2),and Kelch-like family member 15(KLHL15)as their intersecting target genes.The expressions of miR-20a-5p and E2F3 in HF and control groups were significantly different(t=4.289,5.854;P<0.05),while no significant differences were observed in HF patients with various underlying diseases(P>0.05).The ROC curve analysis showed that both miR-20a-5p and E2F3 had diagnostic value for HF,with cut-offvalues of 3.906 and 4.043,respectively.MiR-20a-5p was negatively correlated with BNP and positively correlated with LVEF(r=-0.229,0.337;P<0.05),whereas E2F3 was positively correlated with BNP and negatively correlated with LVEF(r=0.280,-0.277;P<0.05).Conclusion MiR-20a-5p and E2F3 exhibit abnormal expression in HF patients,and both show diagnostic value for HF.关键词
心力衰竭/微小RNA-20a-5p/E2F转录因子3Key words
Heart failure/MicroRNA-20a-5p/E2F transcription factor 3引用本文复制引用
鱼晓铭,郑冠群,盛晓东,朱天哲..微小RNA-20a-5p及E2F转录因子3在心力衰竭患者中的表达情况及临床意义[J].心脑血管病防治,2024,24(12):7-10,28,5.基金项目
常熟市医药科技计划重点项目(CSWS201810) （CSWS201810）
