针刺研究2025，Vol.50Issue(2)：131-140,10.DOI:10.13702/j.1000-0607.20240993
基于PI3K/AKT/NFATc1通路探讨针刀疏筋解结术抑制类风湿关节炎大鼠骨破坏的作用及机制
Effect and mechanism of acupotomy tendon-sparing and knot-dissolving technique in inhibiting bone destruction in rats with rheumatoid arthritis based on the PI3K/AKT/NFATc1 pathway
摘要
Abstract
Objective To observe the effect of acupotomy tendon-sparing and knot-dissolving technique on bone destruction and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/nuclear factor of activated T cells c1(NFATc1)pathway in rats with rheumatoid arthritis(RA),so as to investigate the underlying mechanism.Methods Forty SD rats were randomly divided into normal,model,medication(tripterygium wilfordii),and acupotomy groups,with 10 rats in each group.Except for the normal group,the rest of the rats were injected with bovine type Ⅱ collagen emulsion at the base of tails to establish a collagen-induced RA model.The acupotomy group was treated with acupotomy tendon-sparing and knot-dissolving technique,once every 3 days,with a continuous intervention for 9 times.The medication group was given tripterygium wilfordii polyglycoside suspension(8 mg·kg-1)by gavage,once a day for 28 days continuously.The swelling degree of the ankle joint and the arthritis index score of the rats were observed.Micro-CT scanning was used to observe the degree of bone destruction in the left ankle joint.HE staining and ferruben-solid green staining were used to observe the pathological morphological changes of synovial and cartilage tissue respectively.ELISA was used to detect the contents of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6 and IL-17 in synovial tissue.Tartrate-resistant acid phosphatase(TRAP)staining was used to observe the number of osteoclasts in the left ankle joint.Western blot was used to detect the expression levels of NFATc1,p-PI3K and p-AKT proteins in synovial tissue.Immunohistochemistry was used to detect the positive expressions of matrix metalloproteinase-9(MMP9),cathepsin K(CTSK)and TNF receptor-associated factor 6(TRAF6)in the synovial tissue of the ankle joint.Results In comparison with the normal group,the bones of the ankle joint and toes of rats were severely eroded,with an uneven surface in the model group;there was a large number of inflammatory cell infiltrations in the synovial tissue,obvious damage to the articular cartilage,and disordered arrangement of synovial cells;the cartilage matrix was damaged,the cartilage layer was rough,and the subchondral bone structure was disordered.In comparison with the model group,the above histopathological changes in the medication group and the acupotomy group were alleviated.Compared with the normal group,the joint swelling degree,arthritis index,the ratio of bone surface area to bone volume(BS/BV),trabecular number(Tb.N),trabecular separation(Tb.Sp),the contents of TNF-α,IL-1β,IL-6 and IL-17 in synovial tissue,the number of osteoclasts in the joint,the expressions or ratios of NFATc1,p-PI3K/PI3K,p-AKT/AKT proteins in synovial tissue,and the positive expressions of TRAF6,CTSK and MMP9 proteins in synovial tissue in the model group were significantly increased(P<0.01),while the bone mineral density(BMD),bone volume fraction(BV/TV)and trabecular thickness(Tb.Th)were significantly decreased(P<0.01).Compared with the model group,the joint swelling degree,arthritis index,BS/BV,Tb.Sp,the contents of TNF-α,IL-1β,IL-6 and IL-17 in synovial tissue,the number of osteoclasts,the expressions or ratios of NFATc1,p-PI3K/PI3K,p-AKT/AKT proteins in synovial tissue,and the positive expressions of TRAF6,CTSK and MMP9 proteins in synovial tissue in the medication group and the acupotomy group were significantly decreased(P<0.01,P<0.05),while BMD,BV/TV and Tb.Th were significantly increased(P<0.01,P<0.05),and Tb.N in the acupotomy group was significantly decreased(P<0.01).Conclusion Acupotomy tendon-sparing and knot-dissolving technique can effectively reduce the inflammatory response,relieve the pathological damage of joint tissues and inhibit bone destruction in RA rats,and its mechanism may be related to inhibiting the activation of the PI3K/AKT/NFATc1 pathway.关键词
针刀疏筋解结术/类风湿关节炎/骨破坏/PI3K/AKT/NFATc1通路/破骨细胞Key words
Acupotomy tendon-sparing and knot-dissolving technique/Rheumatoid arthritis/Bone destruction/Phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/nuclear factor of activated T cells c1(NFATc1)pathway/Osteoclast引用本文复制引用
李浩林,景骆羊,陈平,王海东,李伟青,金芳梅,阚丽丽,年芳红,胡乐乐,杨娟娟..基于PI3K/AKT/NFATc1通路探讨针刀疏筋解结术抑制类风湿关节炎大鼠骨破坏的作用及机制[J].针刺研究,2025,50(2):131-140,10.基金项目
国家中医药管理局高水平重点学科建设项目(No.国中医药人教函[2022]226) （No.国中医药人教函[2022]226）
国家自然科学基金项目(No.82460945) （No.82460945）
甘肃省科技计划资助项目(No.24JRRA1044) （No.24JRRA1044）
兰州市指导性计划项目(No.2022-ZD-73) （No.2022-ZD-73）
