中国草食动物科学2025，Vol.45Issue(1)：11-18,8.DOI:10.3969/j.issn.2095-3887.2024.00.114
牛miR-155靶基因预测及其组织表达分析
Target Gene Prediction and Tissue Expression Analysis of Bovine bta-miR-155
摘要
Abstract
To explore the potential function of bta-miR-155 in important tissue of bovine,miRDB,TargetScan and miRWalk online software were used to predict the bta-miR-155 target gene,and the DAVID online database was used to perform GO annotation and KEGG pathway enrichment analysis of the predicted target genes.MEGA 11.0 software was used to analyse conservation for miR-155 from cattle,humans,pigs,dogs,rats,house mice,goats and rhesus ma-caques,and to construct phylogenetic trees.Finally,RT-qPCR was used to detect the expression of bta-miR-155 in the heart,spleen,lung,kidney,testis,biceps femoris and longissimus dorsi muscle of cattle.The results showed that bta-miR-155 was located on chromosome 1 of the bovine genome,and its mature sequence was highly conserved among species,only pigs,mice,and rats were not completely conserved or missing at 12 nt or 23 nt,and there were 24 pre-dicted target genes.GO functional analysis showed that the items with high enrichment of bta-miR-155 target genes mainly included the positive regulation of pri-miRNA transcription by RNA polymerase Ⅱ promoter,the positive regu-lation of NF-kappaB transcription factor activity,the positive regulation of interleukin-1 β production,the positive regulation of smooth muscle cell proliferation,and the negative regulation of myoblast differentiation.The KEGG path-way was significantly enriched in lipid and atherosclerotic pathway,leukocyte differentiation antigen 36,sterol regula-tory element binding protein 1,NOD-like receptor signaling pathway,interleukin-18 and adipocytokine signaling path-way.The results of tissue expression analysis of bta-miR-155 showed that the expression level of bta-miR-155 was the highest in adult bovine biceps femoris muscle and the lowest expression level in myocardium.The expression level of bta-miR-155 in the longissimus dorsi muscle of adult cattle was significantly higher than that in calves(P<0.05).In summary,the high expression of bta-miR-155 in bovine muscle tissue suggests that it may play an important role in muscle growth and development,which lays a foundation for the subsequent exploration of the molecular function of bta-miR-155 in bovine muscle development and myofiber type transformation.关键词
牛/bta-miR-155/靶基因预测/组织表达Key words
bovine/bta-miR-155/target gene prediction/tissue expression分类
畜牧业引用本文复制引用
何学亮,康晓龙,徐俊杰,姬小云,丁燕玲,曲畅,周小南,梁嘉豪,魏大为,史远刚..牛miR-155靶基因预测及其组织表达分析[J].中国草食动物科学,2025,45(1):11-18,8.基金项目
国家自然科学基金项目(32160776) （32160776）
现代农业产业技术体系(CARS-36) （CARS-36）
中国科学院"西部之光"人才培养计划项目(XAB2022YW11) （XAB2022YW11）
宁夏高等教育质量提升工程项目 （）
