中国肺癌杂志2024，Vol.27Issue(12)：885-893,9.DOI:10.3779/j.issn.1009-3419.2024.106.34
电磁脉冲对A549细胞分泌外泌体的影响
Effects of Electromagnetic Pulses on Exosomes Secretion by A549 Cells
摘要
Abstract
Background and objective Numerous researches indicated that electromagnetic pulses(EMP)pos-sessed advantages such as strong targeting,minimal side-effects and low treatment cost in tumor therapy,but its optimum parameters for treatment and the relationship between EMP and tumor-derived exosomes remains unclear.This study aims to clarify the effects of EMP with different parameters on the quantity and miRNA(microRNA)of exosomes secreted by human non-small cell lung cancer A549 cells,providing beneficial reference for the clinical application of EMP and related research.Methods A549 cells were randomly divided into control group and different EMP radiation groups with respective intensity of 400,600 and 800 kV/m.EMP was performed with 2000 pulses once,20 Hz of repetition frequency and 120 ns of pulse width.A549 cells were radiated once per day for continuous 3 days.After radiation,exosomes were collected and identified;cell number was measured by trypan blue staining;the concentration of exosomes was measured by nanoparticle tracking analysis(NTA);the abundance of miRNAs was determined by miRNA sequencing.Results Compared with control group,the mor-phology and cell viability of A549 cells in radiation group was not different,but the quantity of exosomes in 400 or 800 kV/m radiation group was significantly decreased(P<0.05),in contrast with obvious increase in 600 kV/m radiation group(P<0.05).The abundance of exosomal miRNAs between control group and each EMP group was obviously different(P<0.05)and target genes of differentially abundant miRNAs enriched in different pathways.Conclusion Under the experimental condition,the quantity and miRNA abundance of exosomes could be changed by EMP radiation,which could further influence the function of tumor-derived exosomes.关键词
肺肿瘤/A549细胞/外泌体/电磁脉冲/miRNAKey words
Lung neoplasms/A549 cells/Exosomes/Electromagnetic pulses/miRNA引用本文复制引用
侯庆霞,王映梅,曹猛,刘江正,孔德钦,张倩,于卫华,安广洲..电磁脉冲对A549细胞分泌外泌体的影响[J].中国肺癌杂志,2024,27(12):885-893,9.基金项目
本研究受国家自然科学基金项目(No.31900888)资助 This study was supported by the grant from National Natural Science Foundation of China(No.31900888)(to Guangzhou AN). （No.31900888）
