中国免疫学杂志2025，Vol.41Issue(2)：257-262,6.DOI:10.3969/j.issn.1000-484X.2025.02.001
非酒精性脂肪性肝病大鼠模型的造模周期与抗氧化途径的关联研究
Association between modelling cycle and antioxidant pathways in rat models of non-alcoholic fatty liver disease
摘要
Abstract
Objective:To construct a rat model of non-alcoholic fatty liver disease(NAFLD)by choline-deficient high fat diet(CDHFD),and to observe the association between feeding cycle and antioxidant pathway.Methods:The study lasted 16 weeks and was divided into 4 cycles.Detection of pathological changes and expression of antioxidant enzymes in rats liver in different cycles.Results:The early stage of liver steatosis and inflammation in model rats was 2～4 weeks,non-alcoholic steatohepatitis(NASH)stage was 4～8 weeks,and liver fibrosis progression was 8～16 weeks.Mechanistic studies had shown that the expressions of antioxidant enzymes Nrf2,SOD and GSH-Px in the liver of NAFLD rats gradually decreased with the extension of the feeding cycle.Conclusion:Different modeling cycles can successfully induce the pathological changes of steatosis,inflammation and liver fibrosis in rat liver,and the pathological changes are time-dependent with the expressions of antioxidant enzymes.关键词
非酒精性脂肪性肝病(NAFLD)/胆碱缺乏的高脂饮食(CDHFD)/动物模型/抗氧化/Nrf2信号通路Key words
Nonalcoholic fatty liver disease(NAFLD)/Choline-deficient high-fat diet(CDHFD)/Animal models/Antioxi-dant/Nrf2 signaling pathway分类
临床医学引用本文复制引用
郅果果,邵冰杰,冯奕源,朱砂,穆杰,王东..非酒精性脂肪性肝病大鼠模型的造模周期与抗氧化途径的关联研究[J].中国免疫学杂志,2025,41(2):257-262,6.基金项目
国家自然科学基金资助项目(82104655). （82104655）
