中国免疫学杂志2025，Vol.41Issue(2)：271-275,5.DOI:10.3969/j.issn.1000-484X.2025.02.003
Stat1抑制Foxp3的表达和Treg产生
Stat1 inhibits Foxp3 expression and Treg production
摘要
Abstract
Objective:To explore the influence of Stat1 on Foxp3 expression and production of Treg.Methods:C57BL/6 mice were used and separated into normal control group and Stat1 specific inhibitor Fludarabine(Flud)treatment group.Ratio of CD4+Foxp3+Treg and expression of Foxp3 in spleen,lymph nodes and peripheral blood of mice in each group were detected by flow cy-tometry.Human Stat1 overexpression plasmid was constructed and transfected into human breast cancer MCF-7 cells,and expression changes of Foxp3 was detected by RT-qPCR and Western blot.Results:Compared with mice in normal control group,proportion of Treg and expression of Foxp3 in lymph nodes and peripheral blood of mice in Flud treatment group were increased,while Stat1 overex-pression resulted in decreased Foxp3 mRNA and protein expression in MCF-7 cells.Conclusion:Stat1 inhibits expression of Foxp3 and production of Tregs.关键词
Stat1/Foxp3/调节性T细胞/人乳腺癌细胞/MCF-7Key words
Stat1/Foxp3/Treg/Human breast cancer cell/MCF-7分类
基础医学引用本文复制引用
郭帅,张格,陈昊,秦安琪,李文婷,张爱红,郑爱华,田枫,郑全辉..Stat1抑制Foxp3的表达和Treg产生[J].中国免疫学杂志,2025,41(2):271-275,5.基金项目
国家自然科学基金面上项目(81373111) （81373111）
河北省卫生健康委资助项目(20190105). （20190105）
