中国免疫学杂志2025，Vol.41Issue(2)：276-280,286,6.DOI:10.3969/j.issn.1000-484X.2025.02.004
肺泡巨噬细胞对屋尘螨诱导的过敏性气道炎症的影响
Effects of alveolar macrophages on allergic airway inflammation induced by house dust mites
摘要
Abstract
Objective:To study the changes of immune cells in allergic airway inflammation induced by house dust mites(HDM)in the mouse model,and investigate the effect of alveolar macrophages(AM)on allergic airway inflammation.Methods:Al-lergic airway inflammation was induced by intratracheal injection of HDM,and AM were specifically depleted by intratracheal injec-tion of chlorophosphonate liposomes(CL)before HDM treatment;the immune cells of mouse lung were isolated by heart perfusion.The immune cells of mouse alveolar lavage fluid were isolated by tracheal lavage.The level of type Ⅱ cytokines in alveolar lavage fluid was determined by ELISA.The changes of immune cells in mouse lung tissue and alveolar lavage fluid were detected by flow cytome-try;HE staining and PAS staining were performed to observe the infiltration of inflammatory cell,goblet cell proliferation and mucus secretion in the lung sections after HDM treatment.Results:The effector cells of type Ⅱ immune response,such as group 2 innate lymphoid cells(ILC2)and eosinophils,were significantly increased in allergic airway inflammation induced by HDM in mice.Histo-logical analysis also revealed substantial inflammatory cell infiltration,goblet cells proliferation and mucus secretion in lung tissue.The depletion of AM alleviated HDM induced type Ⅱ immune response,but promoted the infiltration of neutrophils and aggravated the pathological injury of the lung.Conclusion:AM plays an important role in HDM induced allergic airway inflammation.关键词
屋尘螨/过敏性气道炎症/肺泡巨噬细胞/嗜酸性粒细胞/Ⅱ型先天性淋巴细胞Key words
House dust mites/Allergic airway inflammation/Alveolar macrophages/Eosinophil/Group 2 innate lymphoid cells分类
基础医学引用本文复制引用
程百合,杨文婷,侯飞,唐丽..肺泡巨噬细胞对屋尘螨诱导的过敏性气道炎症的影响[J].中国免疫学杂志,2025,41(2):276-280,286,6.基金项目
国家自然科学基金青年基金(31900632). （31900632）
