中国免疫学杂志2025，Vol.41Issue(2)：295-303,9.DOI:10.3969/j.issn.1000-484X.2025.02.007
慢性阻塞性肺疾病大鼠模型CD8+T细胞自噬水平研究及与其数量的相关性分析
Autophagy level of CD8+T cells in rat model of chronic obstructive pulmonary disease and correlation with its number
摘要
Abstract
Objective:To investigate the autophagy level of CD8+T cells in rat model of chronic obstructive pulmonary disease(COPD)and correlation with its number,and to explore the role of autophagy in pathogenesis of COPD.Methods:Thirty-six 6-week-old male SD rats were divided into three groups,including normal control group,COPD group and COPD intervention group.COPD model was established by smoking,and intraperitoneal injection of 3-methyladenine(3-MA)was used for intervention.Lung function of rats was detected by small animal pulmonary function testing,and frequency of CD8+T cells,CD8+memory T cells,CD8+effector T cells in blood and lung of rats were detected by flow cytometry.CD8+T cells were sorted by immunomagnetic beads,and expressions of autophagy protein including LC3-Ⅱ/Ⅰ,Beclin-1 and p62 in CD8+T cells were detected by Western blot.The middle lobe of right lung was collected for histological observation.Results:Infiltration of inflammatory cells and the decline of lung function in COPD group were more obvious than those in normal group.Frequency of CD8+T cells,CD8+effector T cells and CD8+memory T cells in blood and lung of rats in COPD group were significantly higher than those in normal control group(all P<0.05).Level of autophagy protein,LC3-Ⅱ/Ⅰ and Beclin-1 of CD8+T cells in COPD group were significantly higher than that in normal control group,while level of p62 was lower than that in normal control group(P<0.01).CD8+T cells,CD8+effector T cells,CD8+memory T cells in COPD intervention group were significantly lower than those in COPD group(P<0.05).Correlation analysis showed that LC3-Ⅱ/Ⅰ expression level was positively correlated with frequency of CD8+T cells in blood(r=0.667,P<0.01).Expression level of Beclin-1 was positively correlated with frequency of CD8+T cells and CD8+effector T cells in blood(r=0.505,P=0.021;r=0.428,P=0.037).Expression of LC3-Ⅱ/Ⅰprotein was positively correlated with frequency of CD8+T cells and CD8+effector T cells in lung tissue(r=0.474,P=0.019;r=0.549,P=0.006).Expression of Beclin-1 was positively correlated with frequency of CD8+effector T cells in lung tissue(r=0.458,P=0.025).Conclusion:Level of CD8+T cell autophagy is correlated with its number.CD8+T cell autophagy may be involved in the chronic inflam-matory process of COPD,and 3-MA can inhibit COPD inflammation.关键词
慢性阻塞性肺疾病/CD8+T细胞/自噬/3-甲基腺嘌呤Key words
Chronic obstructive pulmonary disease/CD8+T cells/Autophagy/3-methyladenine分类
临床医学引用本文复制引用
陈宇鸣,于旭华,梁紫尧,李娜,陈思达,申严,卓宋明..慢性阻塞性肺疾病大鼠模型CD8+T细胞自噬水平研究及与其数量的相关性分析[J].中国免疫学杂志,2025,41(2):295-303,9.基金项目
深圳市科技计划基础研究项目(JCYJ20160427100015608). （JCYJ20160427100015608）
