中国免疫学杂志2025，Vol.41Issue(2)：315-319,5.DOI:10.3969/j.issn.1000-484X.2025.02.010
巨噬细胞极化对血管平滑肌细胞PI3K/Akt/mTOR信号通路的影响
Effect of macrophage polarization on PI3K/Akt/mTOR signaling pathway in vascular smooth muscle cells
摘要
Abstract
Objective:To investigate effects of macrophage polarization on PI3K/Akt/mTOR signaling pathway and inflammatory response of vascular smooth muscle cells.Methods:THP-1 cells were induced to become macrophages by phaboate,then treated with LPS and IFN-γ,IL-4 and IL-13 for 48 h,and cultured with fresh medium without serum for 24 h.Supernatant was used as conditioned medium.Vascular smooth muscle cells were divided into control group,M0 medium group,M1 medium group and M2 medium group.CCK-8 was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,ELISA was used to detect expressions of inflammatory cytokines IL-1α,IL-6 and TGF-β in supernatant of cells,and mRNA and phosphorylated protein expressions of PI3K,Akt and mTOR in vascular smooth muscle cells were detected by RT-qPCR and Western blot.Results:Compared with control group,cell proliferation ability and TGF-β level in supernatant of M0 medium group were significantly decreased(P<0.01),apoptosis rate,IL-1α and IL-6 levels in cell supernatant,mRNA and protein phosphorylation levels of PI3K,Akt and mTOR in cells were significantly increased(P<0.01).Compared with M0 medium group,cell proliferation ability and TGF-β level in supernatant of M1 medium group were significantly decreased(P<0.05),apoptosis rate,IL-1α and IL-6 levels in cell supernatant,mRNA and protein phosphorylation levels of PI3K,Akt and mTOR in cells were significantly increased(P<0.01),the trend was opposite in M2 medium group(P<0.05).Conclusion:Macrophage polarization can regulate expressions of inflammatory cytokines by regulating PI3K/Akt/mTOR signaling pathway,and participate in inflammatory response in atherosclerosis.关键词
巨噬细胞极化/动脉粥样硬化/PI3K/Akt/mTOR信号通路Key words
Macrophage polarization/Atherosclerosis/PI3K/Akt/mTOR signaling pathway分类
基础医学引用本文复制引用
冯莹,张妍,雷杰,刘娟,方勇,贺立群..巨噬细胞极化对血管平滑肌细胞PI3K/Akt/mTOR信号通路的影响[J].中国免疫学杂志,2025,41(2):315-319,5.基金项目
武汉市卫健委青年基金(WX21Q11). （WX21Q11）
