中国免疫学杂志2025，Vol.41Issue(2)：328-335,8.DOI:10.3969/j.issn.1000-484X.2025.02.012
NRIP1通过转录激活HMGB1参与脓毒症引起的肠道上皮细胞损伤的作用研究
Effect of NRIP1 on participating in sepsis-induced intestinal epithelial injury via transcriptional activation of HMGB1
摘要
Abstract
Objective:To investigate the impacts of nuclear receptor-interacting protein 1(NRIP1)on sepsis-evoked intesti-nal epithelial injury via transcriptional regulation of high mobility group box 1(HMGB1).Methods:The expression levels of NRIP1 and HMGB1 were detected by RT-qPCR and Western blot.The pathological changes of intestinal tissue were detected by HE staining.CCK-8 assay determined the optimal treatment time of LPS.Caco-2 cells were transfected with NRIP1 small interfering RNA(siRNA-NRIP1-1/2),and cell viability and apoptosis were detected by CCK-8 assay and flow cytometry,respectively.RT-qPCR and Western blot examined the expressions of inflammation-associated factors.Transepithelial resistance(TEER)was used to detect intestinal epi-thelial permeability.Western blot was used to detect the expressions of apoptosis and tight-junction related proteins.The binding rela-tionship between NRIP1 and HMGB1 was verified by luciferase reporting assay and chromatin immunoprecipitation assay(ChIP).After knocking down NRIP1 and overexpressing HMGB1 in LPS-treated Caco-2 cells,the functional experiment was performed again.Results:NRIP1 expression was fortified in the intestinal tissues of sepsis rats and LPS-treated Caco-2 cells.Interference with NRIP1 attenuated LPS-elicited Caco-2 cell viability injury,apoptosis,inflammatory response and barrier damage.Additionally,NRIP1 might activate HMGB1 expression at transcriptional level and HMGB1 elevation might reverse the impacts of NRIP1 absence on Caco-2 cell viability,apoptosis,inflammatory response as well as barrier function.Conclusion:NRIP1 may promote sepsis-elicited intestinal epi-thelial injury,which may be related to transcriptional activation of HMGB1.关键词
NRIP1/HMGB1/脓毒症/肠道上皮细胞损伤Key words
NRIP1/HMGB1/Sepsis/Intestinal epithelial injury分类
医药卫生引用本文复制引用
崔文娟,刘芹,樊晓光,乔鲁军..NRIP1通过转录激活HMGB1参与脓毒症引起的肠道上皮细胞损伤的作用研究[J].中国免疫学杂志,2025,41(2):328-335,8.基金项目
山东省医药卫生科技发展计划项目,黄河三角洲学者专项经费资助(202117010420). （202117010420）
