中国免疫学杂志2025，Vol.41Issue(2)：351-356,6.DOI:10.3969/j.issn.1000-484X.2025.02.015
膀胱癌细胞外泌体诱导中性粒细胞外诱捕网形成对肿瘤细胞增殖、迁移和侵袭的研究
Bladder cancer exosomes induce formation of neutrophil extracellular traps to promote proliferation,migration and invasion of tumor cells
摘要
Abstract
Objective:To investigate the function and mechanism of exosomes in bladder cancer cell growth and metastasis regulating by NETs.Methods:The exosomes secreted by SV-HUC-1,MGH-U3 and 253J cells were extracted.PBS and the above exo-somes incubated with PMN,recorded as PBS group,SV-HUC-1 exo group,MGH-U3 exo group,253J exo group.The generation of NETs was detected by Sytox green staining,and the expressions of NETs marker proteins CIT-H3,NE and MPO were detected by Western blot.MGH-U3 and 253J cells were divided into Control group,PMN group,MGH-U3 exo group,253J exo group MGH-U3 exo+PMN group,253J exo+PMN group.The PMN culture supernatant of MGH-U3 exo group and 253J exo group were added with 1.5 U/ml DNAse,after incubation for 30 min,the collected mixture to co-incubate with MGH-U3 and 253J cells,recorded as MGH-U3 exo+PMN+DNAse group and 253J exo+PMN+DNAse group.The proliferation ability of MGH-U3 and 253J cells were detected by CCK8,the migration and invasion of cells were detected by Transwell,and the phosphorylation levels of PI3K and AKT were detected by Western blot.Results:Compared with PBS group,the formation of NETs and the expressions of CIT-H3,NE and MPO in MGH-U3 exo group and 253J exo group increased significantly(P<0.001).There was no significant change of the above indexes in SV-HUC-1 exo group.Compared with Control group,MGH-U3 exo+PMN group and 253J exo+PMN group had significantly increased cell prolifer-ation,migration and invasion ability(P<0.05),the phosphorylation levels of PI3K and AKT increased significantly(P<0.001).Com-pared with MGH-U3 exo+PMN group,MGH-U3 exo+PMN+DNAse group had significantly decreased cell proliferation,migration and invasion ability(P<0.05),the phosphorylation levels of PI3K and AKT decreased significantly(P<0.001).Compared with 293J exo+PMN group,293J exo+PMN+DNAse group had significantly decreased cell proliferation,migration and invasion ability,the phosphor-ylation levels of PI3K and AKT decreased significantly(P<0.001).Conclusion:The exosomes of bladder cancer cells can promote the proliferation,migration and invasion of tumor cells by inducing the generation of NETs.关键词
膀胱癌/外泌体/NETs/增殖/迁移/侵袭Key words
Bladder cancer/Exosome/NETs/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
符仕宝,陈宁,何书明,杨杰,彭泽椿,徐明彬..膀胱癌细胞外泌体诱导中性粒细胞外诱捕网形成对肿瘤细胞增殖、迁移和侵袭的研究[J].中国免疫学杂志,2025,41(2):351-356,6.基金项目
海南省卫生健康行业科研项目(22A200154). （22A200154）
