中国免疫学杂志2025，Vol.41Issue(2)：362-366,373,6.DOI:10.3969/j.issn.1000-484X.2025.02.017
肺癌细胞外泌体诱导M2型巨噬细胞的极化促进肺癌细胞迁移和侵袭作用及其机制研究
Function and mechanism of lung cancer cell exosomes induce polarization of M2 macrophages to promote lung cancer cell migration and invasion
摘要
Abstract
Objective:To investigate the effect of exosomes from lung cancer cells on the ability of lung cancer metastasis in-duced by M2 macrophages.Methods:The exosomes secreted by 16HBE,A549 and H522(16HBE exo,A549 exo,H522 exo)were extracted and identified.M0 macrophages co-incubated with 10 µg/ml 16HBE exo,A549 exo,H522 exo,PBS(16HBE exo group,A549 exo group,H522 exo group,PBS group),the expressions of Arginase-1,CD206,CD163,TGF-β,iNOS,and IL-1β in macro-phages in each group were detected by qRT-PCR,the proportion of CD206+CD163+macrophages was detected by flow cytometry,the phosphorylation levels of ERK1/2 and STAT3 in macrophages were detected by Western blot.A549 and H522 cells were co-incubated with macrophages in the above groups(16HBE exo+M0 group,A549 exo+M0 group,H522 exo+M0 group,PBS+M0 group),and the migration and invasive ability of A549 and H522 cells in each group were detected by Transwell chamber method.Results:Compared with PBS group,the expressions of Arginase-1,CD206,CD163 and TGF-β in macrophages in A549 exo group and H522 exo group were significantly up-regulated(P<0.001),and the expressions of iNOS and IL-1β were significantly down-regulated(P<0.001).The proportion of CD206+CD163+macrophages was increased significantly(P<0.001),and the phosphorylation levels of ERK1/2 and STAT3 were increased significantly(P<0.001).There was no significant difference in the above indicators between the 16HBE exo group and the PBS group(P>0.05).The migration ablility and invasion ablility of A549 and H522 cells in A549 exo+M0 group and H522 exo+M0 group were significantly increased(P<0.001).Conclusion:Lung cancer cell exosomes can induce the polarization of M2 macrophages by activating the ERK1/2/STAT3 signaling pathway to promote tumor migration and invasion.关键词
肺癌/外泌体/M2型巨噬细胞/迁移/侵袭Key words
Lung cancer/Exosomes/M2 macrophages/Migration/Invasion分类
医药卫生引用本文复制引用
周薇,李金权,马文卓,王有德,童红梅..肺癌细胞外泌体诱导M2型巨噬细胞的极化促进肺癌细胞迁移和侵袭作用及其机制研究[J].中国免疫学杂志,2025,41(2):362-366,373,6.基金项目
甘肃省科技计划项目(20JR5RA170). （20JR5RA170）
