中国农学通报2025，Vol.41Issue(3)：59-68,10.
不同栽培模式下多花黄精根腐病病株与健株根际土壤真菌多样性分析
Analysis of Rhizosphere Soil Fungal Diversity Between Root Rot Diseased and Healthy Polygonatum cyrtonema Plants Under Different Cultivation Modes
摘要
Abstract
The changes of community structure and fungal diversity in the rhizosphere soil of healthy and root rot diseased Polygonatum cyrtonema under varied cultivation modes were studied to provide theoretical support for the rational cultivation of P.cyrtonema and the targeted improvement of soil microbial community.Based on the Illumina HiSeq sequencing platform,the Paired-end was used to systematically analyze the changes of community structure and fungal diversity in the rhizosphere soil of healthy and root rot diseased P.cyrtonema that planted in the field and understory.The results showed that Ascomycota and Basidiomycota were the dominant fungal phyla in the rhizosphere soil of healthy and diseased plants under different cultivation patterns.Compared with the field cultivation,the rhizosphere soil fungal amount and the relative abundance of Ascomycota(except 4a diseased samples)were significantly increased in understory cultivation,while the relative abundance of Basidiomycota and Fusarium genera(except 4a diseased samples)were significantly decreased.Except for the 2a healthy plants,the rhizosphere soil fungal diversity of P.cyrtonema planted in understory was significantly higher than that in field.Among them,the Ace index and Chao1 index of the 2a diseased plants,4a healthy plants and 4a diseased plants in the understory cultivation were 9.09%,20.95%,24.22%and 24.36%,12.19%and 12.27%higher than those in field,respectively.Compared with healthy plants,the rhizosphere soil fungal amount of 2a diseased plants in field and 4a diseased plants in understory were significantly reduced,but the relative abundance of Fusarium genera in the rhizosphere soil of 4a diseased plants was significantly increased.There were no significant differences in the alpha diversity index between healthy and diseased P.cyrtonema under different cultivation modes(P＞0.05).In conclusion,there were large differences in the rhizosphere soil fungal community structure and fungal diversity between healthy and diseased P.cyrtonema plants under different cultivation modes,and the relative abundance of harmful fungal genera,such as Fusarium in the rhizosphere soil,was one of the important reasons for the occurrence of P.cyrtonema root rot.关键词
多花黄精/土壤真菌多样性/根腐病/林下栽培/大田种植/Illumina HiSeq测序Key words
Polygonatum cyrtonema/soil fungal diversity/root rot/understory cultivation/field cultivation/Illumina HiSeq sequencing分类
农业科技引用本文复制引用
戈秀梅,李芳,万晓婷,韩邦兴,陈存武..不同栽培模式下多花黄精根腐病病株与健株根际土壤真菌多样性分析[J].中国农学通报,2025,41(3):59-68,10.基金项目
安徽省现代中药产业共性技术研究中心"大别山区多花黄精产业化开发关键技术研究与应用"(GXXT-2022-087). （GXXT-2022-087）
