中国农学通报2025，Vol.41Issue(3)：107-113,7.
水蜡A病毒丁香分离物的鉴定与全基因组序列分析
Ligustrum Virus A Isolates from Syringa lindl:Identification and Whole Genome Sequence Analysis
摘要
Abstract
To identify and characterize the candidate viruses infecting Beijing lilac(Syringa lindl)associated with the symptoms of leaf chlorosis,mosaic and leaf curling,small RNA deep sequencing and RT-PCR were conducted with the diseased leaf samples.Ligustrum virus A(LVA)was detected and the complete genome sequences of two isolates were obtained.The Beijing lilac isolate LVA-NXY is 8488 nt in length,while the isolate LVA-HLG is 8478 nt.Sequence similarity analysis showed that LVA-NXY and LVA-HLG were most similar to the LVA-DBLQ isolate from Forsythia suspensa in Shenyang,China.No recombination events were detected among the seven reported LVA isolates.The phylogenetic tree based on the genome sequences indicated that LVA-NXY and LVA-HLG clustered together with LVA-DBLQ and the Syringa vulgaris isolate LVA-DX in the same branch,showing a close phylogenetic relationship.This study reports the complete genome sequence of ligustrum virus A infecting Beijing lilac and explores the molecular characteristics and evolutionary relationships of the virus which provides a theoretical basis for the study of genetic variation and the control of lilac virus disease.关键词
丁香/水蜡A病毒/小RNA/基因组/序列分析/分子特征/进化关系Key words
Syringa lindl/Ligustrum virus A/small RNA/genome/sequence analysis/molecular characteristics/phylogenetic relationship分类
农业科技引用本文复制引用
王昀轩,刘丽,孙春辉,鲁书豪,梁宇卿,崔雯莉,高佳仪,李永强..水蜡A病毒丁香分离物的鉴定与全基因组序列分析[J].中国农学通报,2025,41(3):107-113,7.基金项目
北京市教委科研项目"利用siRNA序列组装技术鉴定葡萄新病毒"(KM201610020008). （KM201610020008）
