中国农业科技导报2025，Vol.27Issue(2)：24-32,9.DOI:10.13304/j.nykjdb.2023.0260
CRISPR-Cas系统在枯草芽孢杆菌基因组编辑中的研究进展
Recent Advances of CRISPR-Cas System in Genome Editing of Bacillus subtilis
摘要
Abstract
Bacillus subtilis is a food safety microorganism,which has been widely used in industrial fermentation.CRISPR(clustered regularly interspaced short palindromic repeats)mediated genome editing technology has played an important role in the research of microbial metabolic engineering with B.subtilis as the chassis cell.The immune response mechanism and classification of CRISPR-Cas system were introduced,as well as the 3 types of CRISPR-Cas9 genome editing in B.subtilis.It focused on summarizing the latest CRISPR development and design strategies,with a view to providing references for optimizing existing B.subtilis genome editing systems,thereby improving the industrial application potential of B.subtilis.关键词
枯草芽孢杆菌/基因编辑/CRISPR-Cas9/CRISPR-Cpf1/PAM无依赖Key words
Bacillus subtilis/genome editing/CRISPR-Cas9/CRISPR-Cpf1/PAM-independent分类
生物工程引用本文复制引用
孙志康,李力群,郝捷,吴晗,吴娜,郑超,季嫱,李选文,陈晨..CRISPR-Cas系统在枯草芽孢杆菌基因组编辑中的研究进展[J].中国农业科技导报,2025,27(2):24-32,9.基金项目
中国烟草总公司重点研发项目(No.110202102019). （No.110202102019）
