不同菜用甘薯品种茎尖代谢产物鉴定及途径分析OA北大核心
Separation and Identification of Metabolites and Metabolic Pathway Analysis in Different Vegetable Sweet Potato
为研究代谢产物对甘薯生长发育的影响,以甘薯茎尖为研究对象,利用液相色谱-质谱联用(liquid chromatograph-mass spectrometer,LC-M)分离鉴定南宁菜、福菜薯18号、桂菜薯1号、桂菜薯2号、福薯7-6和台农71共6种菜用甘薯的差异代谢产物,探讨代谢途径的差异.结果表明,6个甘薯品种共得到差异代谢物4 210种,其中上调2 229种,下调1 981种,表明不同甘薯品种间存在显著差异.差异代谢物涉及的代谢途径主要包括初级胆汁酸生物合成、吲哚生物碱生物合成、黄酮和黄酮醇生物合成和色氨酸代谢,且不同品种间差异代谢物的积累和代谢途径也存在较大差异.因此,推测差异代谢物的积累和代谢途径的差异对甘薯品质起着关键作用.以上研究结果为进一步分析不同品种甘薯品质差异的机制奠定了基础.
To study the effects of metabolites on the growth and development of vegetable sweet potato,the stem tips of sweet potatoes were as materials,and liquid chromatograph-mass spectrometer(LC-MC)was employed to identify and separate metabolic products of 6 sweet potato cultivars including Nanningcai,Fucaishu 18,Guicaishu 1,Guicaishu 2,Fushu 7-6 and Tainong 71.The differences of metabolic pathways were further discussed.The results revealed a total of 4 210 differential metabolites were detected across 6 sweet potato cultivars,with 2 229 up-regulated and 1 981 down-regulated,which showed that there were significant differences among different sweet potato cultivars.The primary metabolic pathways associated with differential metabolism included primary bile acid biosynthesis,indole alkaloid biosynthesis,flavonoid and flavonol biosynthesis and tryptophan metabolism.The accumulation of differential metabolites and their respective metabolic pathways among different varieties had significant differences.Consequently,it was hypothesized that the differences in both the accumulation and metabolism of these metabolites played a crucial role in sweet potato quality.Above results provided reliable basis for understanding the mechanisms of differences on quality of sweet potato.
张奕萱;李慧峰;黄咏梅;李彦青;滑金锋;银捷;陈天渊;肖冬;莫云川
广西大学农学院,广西农业环境与农产品安全重点实验室,南宁 530004广西壮族自治区农业科学院玉米研究所,南宁 530007||广西壮族自治区农业科学院旱粮作物研究所,南宁 530007广西壮族自治区农业科学院玉米研究所,南宁 530007||广西壮族自治区农业科学院旱粮作物研究所,南宁 530007广西壮族自治区农业科学院玉米研究所,南宁 530007||广西壮族自治区农业科学院旱粮作物研究所,南宁 530007广西壮族自治区农业科学院玉米研究所,南宁 530007||广西壮族自治区农业科学院旱粮作物研究所,南宁 530007广西壮族自治区农业科学院玉米研究所,南宁 530007||广西壮族自治区农业科学院旱粮作物研究所,南宁 530007广西壮族自治区农业科学院玉米研究所,南宁 530007||广西壮族自治区农业科学院旱粮作物研究所,南宁 530007广西大学农学院,广西农业环境与农产品安全重点实验室,南宁 530004广西大学农学院,广西农业环境与农产品安全重点实验室,南宁 530004
农业科学
菜用甘薯茎尖代谢产物代谢途径
vegetable sweet potatostem tipmetabolitemetabolic pathway
《中国农业科技导报》 2025 (2)
62-69,8
国家自然科学基金项目(32060419,32060469)国家甘薯产业技术体系建设项目(CARS-10-SYZ14)广西农业科学院基本科研业务专项(桂农科2021YT022,桂农科2023YM02).
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