中国农业科技导报2025，Vol.27Issue(2)：125-135,11.DOI:10.13304/j.nykjdb.2023.0647
番茄黄化曲叶病毒胁迫下外源NO对番茄抗氧化物酶基因表达的影响
Effect of Exogenous NO on Expression of Tomato Antioxidant Enzyme Gene Under Tomato Yellowing Leaf Curl Virus Stress
摘要
Abstract
In order to investigate the effect of exogenous nitric oxide(NO)on the expression of tomato antioxidant gene under the stress of tomato yellow leaf curl virus(TYLCV),the susceptible tomato Jinpeng 1 was used as the experimental material.Transcriptome sequencing,fluorescent qRT-PCR and bioinformatics analysis were performed under control(CK),TYLCV(TY)and NO+TYLCV(NO+TY)treatments.The results showed that a total of 55 antioxidase-coding genes were selected in the tomato genome,among which the antioxidase-containing genes with the highest number of exons in different subcellular regions were significantly responsive to TYLCV stress.The number of NO-mediated antioxidant enzyme coding genes in different subcellular compartms showed chloroplast>cell membrane>cytoplasm>peroxisome>vacuole,in which the expression levels of Chl Cu-Zn SOD,Chl MR2,Chl GR,Per MR,Pla CAT1 and Pla CAT7 were significantly up-regulated,and the expression levels of Chl Fe SOD1,Chl Fe SOD2,Cyt GPX,Cyt APX1,Cyt APX2 L-5,Pla CAT3,Pla CAT8 and Vac CAT were significantly down-regulated.Fluorescence qRT-PCR verified that Chl GR,Min Mn SOD and Per CAT2 responded to TYLCV,and Chl Cu-Zn SOD,Pla CAT7,Pla CAT8 and Cyt APX2 L-5 responded to TYLCV and NO.Above results provided theoretical basis for studying the mechanism of NO in improving tomato disease resistance.关键词
番茄/番茄黄花曲叶病/一氧化氮/抗氧化酶基因Key words
tomato/tomato yellow leaf curl virus/nitric oxide/antioxidant enzyme gene分类
农业科技引用本文复制引用
罗金城,朱晓林,魏小红,王贤,王宝强,杜雪芬..番茄黄化曲叶病毒胁迫下外源NO对番茄抗氧化物酶基因表达的影响[J].中国农业科技导报,2025,27(2):125-135,11.基金项目
国家自然科学基金项目(32060401). （32060401）
