低氧胁迫对绿鳍马面鲀血液生理生化、鳃组织结构和肝脏抗氧化能力的影响OA北大核心CSTPCD
Effects of hypoxia stress on hematological physiology and biochemis-try,gill histiology and hepatic antioxidant capacity of black scraper Thamnaconus septentrionalis
为探究绿鳍马面鲀(Thamnaconus modestus)的低氧耐受能力和低氧胁迫过程中血液生理生化指标、鳃组织结构和肝脏抗氧化能力的变化情况.本研究使用封闭静水法,分析了绿鳍马面鲀临界氧分压(critical oxygen tension,Pcrit)和失去平衡点(loss of equilibrium,LOE)时的水体溶解氧浓度,随后分别在Pcrit、LOE、恢复正常溶解氧3 h(R3)和6 h(R6)时,检测绿鳍马面鲀血液生理生化指标、鳃组织结构和肝脏抗氧化酶活力的变化.结果显示,绿鳍马面鲀[(101.23±14.28)g]在Pcrit和LOE时的水体溶解氧浓度分别为(4.0±0.2)mg/L和(2.2±0.2)mg/L.低氧胁迫下,血液葡萄糖和皮质醇浓度显著升高,白细胞数目和红细胞数目在LOE时显著升高(P＜0.05),血红蛋白浓度和红细胞压积在LOE时呈上升趋势,但变化不显著(P＞0.05).鳃小片的长度、宽度、基质厚度和周长伴随着低氧胁迫进程显著增加,鳃小片间距显著减小,气体交换率显著降低(P＜0.05);鳃小片呈现末端杵状、融合和基质增生.肝脏组织中的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性以及丙二醛含量在低氧胁迫后均显著升高(P＜0.05),在LOE时达到最大值.恢复正常溶解氧后,血液生理生化指标、鳃组织结构和肝脏抗氧化酶活力逐渐恢复或接近正常水平,在恢复6 h后上述大部分指标与对照组无显著差异.结果表明,低氧应激导致绿鳍马面鲀血液生理生化相关指标、鳃小片组织形态和肝脏抗氧化能力发生显著改变,恢复正常溶解氧6 h后低氧胁迫诱导的生理改变和潜在的应激损伤显著缓解.相关结果为研究绿鳍马面鲀低氧耐受生理调控机制和高效健康养殖提供了基础理论依据和数据支撑.
In the present study,the hypoxia tolerance and alternation of hematological,physiological,and biochemical parameters,gill histology,and hepatic antioxidant capacity subjected to hypoxia stress were investigated in black scraper Thamnaconus modestus.The closed static water method was used to analyze the dissolved oxygen(DO)levels at critical oxygen tension(Pcrit)and loss of equilibrium(LOE)in black scraper.Subsequently,changes in hematological,physiological,and biochemical parameters,gill histology,and hepatic antioxidant enzyme activity were detected at Pcrit,LOE,reoxygenation 3 hours(R3)and 6 hours(R6).The results showed that the DO concentrations of the black scraper[(101.23±14.28)g]at Pcrit and LOE were(4.0±0.2)mg/L and(2.2±0.2)mg/L,respectively.Hypoxia significantly increased glucose and cortisol levels.White and red blood cell numbers also increased with the highest value observed at LOE(P＜0.05).The hemoglobin concentration and hematocrit showed an upward trend at LOE,but the difference was not significant(P＞0.05).Meanwhile,hypoxia significantly increased the length,width,basal epithelial thickness,and perimeter of the secondary lamellae,and decreased interlamellar distance and the proportion of available gas exchange(P＜0.05).Hypoxia stress also induced branchial clubbing,fusion,and matrix hyperplasia of the secondary lamellae.Hepatic superoxide dismutase,catalase,glutathione peroxidase activity,and malondialdehyde levels significantly increased under hypoxia(P＜0.05),peaking at LOE.Reoxygenation treatment for 6 hours alleviated the stress mentioned above effects.These results indicated that hypoxia stress lead to significantly alterations in the black scraper's hematological,physiological and biochemical profiles,gill morphology,and hepatic antioxidant capacity,which are substantially alleviated after 6 hours re-oxygenation.These findings provide valuable insights into the physiological mechanisms underlying hypoxia tolerance and support the development of efficient and healthy breeding practices for black scraper.
刘志飞;汪峰;陈帅钰;陈夏添;贾玉东
中国水产科学研究院黄海水产研究所,山东青岛 266071||浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山 316022中国水产科学研究院黄海水产研究所,山东青岛 266071中国水产科学研究院黄海水产研究所,山东青岛 266071中国水产科学研究院黄海水产研究所,山东青岛 266071中国水产科学研究院黄海水产研究所,山东青岛 266071||浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山 316022||青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室,山东青岛 266237
水产学
绿鳍马面鲀低氧胁迫血液生理生化鳃组织结构肝脏抗氧化能力
Thamnaconus modestushypoxia stressblood physiology and biochemistrygill tissue structureliver antioxidant capacity
《中国水产科学》 2024 (12)
1441-1452,12
中央支持地方科技成果转化项目(YDZX2022120)中国水产科学研究院基本科研业务费项目(2023TD81,20603022023018).
评论