中国水产科学2024，Vol.31Issue(12)：1441-1452,12.DOI:10.12264/JFSC2024-0220
低氧胁迫对绿鳍马面鲀血液生理生化、鳃组织结构和肝脏抗氧化能力的影响
Effects of hypoxia stress on hematological physiology and biochemis-try,gill histiology and hepatic antioxidant capacity of black scraper Thamnaconus septentrionalis
摘要
Abstract
In the present study,the hypoxia tolerance and alternation of hematological,physiological,and biochemical parameters,gill histology,and hepatic antioxidant capacity subjected to hypoxia stress were investigated in black scraper Thamnaconus modestus.The closed static water method was used to analyze the dissolved oxygen(DO)levels at critical oxygen tension(Pcrit)and loss of equilibrium(LOE)in black scraper.Subsequently,changes in hematological,physiological,and biochemical parameters,gill histology,and hepatic antioxidant enzyme activity were detected at Pcrit,LOE,reoxygenation 3 hours(R3)and 6 hours(R6).The results showed that the DO concentrations of the black scraper[(101.23±14.28)g]at Pcrit and LOE were(4.0±0.2)mg/L and(2.2±0.2)mg/L,respectively.Hypoxia significantly increased glucose and cortisol levels.White and red blood cell numbers also increased with the highest value observed at LOE(P＜0.05).The hemoglobin concentration and hematocrit showed an upward trend at LOE,but the difference was not significant(P＞0.05).Meanwhile,hypoxia significantly increased the length,width,basal epithelial thickness,and perimeter of the secondary lamellae,and decreased interlamellar distance and the proportion of available gas exchange(P＜0.05).Hypoxia stress also induced branchial clubbing,fusion,and matrix hyperplasia of the secondary lamellae.Hepatic superoxide dismutase,catalase,glutathione peroxidase activity,and malondialdehyde levels significantly increased under hypoxia(P＜0.05),peaking at LOE.Reoxygenation treatment for 6 hours alleviated the stress mentioned above effects.These results indicated that hypoxia stress lead to significantly alterations in the black scraper's hematological,physiological and biochemical profiles,gill morphology,and hepatic antioxidant capacity,which are substantially alleviated after 6 hours re-oxygenation.These findings provide valuable insights into the physiological mechanisms underlying hypoxia tolerance and support the development of efficient and healthy breeding practices for black scraper.关键词
绿鳍马面鲀/低氧胁迫/血液生理生化/鳃组织结构/肝脏抗氧化能力Key words
Thamnaconus modestus/hypoxia stress/blood physiology and biochemistry/gill tissue structure/liver antioxidant capacity分类
农业科技引用本文复制引用
刘志飞,汪峰,陈帅钰,陈夏添,贾玉东..低氧胁迫对绿鳍马面鲀血液生理生化、鳃组织结构和肝脏抗氧化能力的影响[J].中国水产科学,2024,31(12):1441-1452,12.基金项目
中央支持地方科技成果转化项目(YDZX2022120) （YDZX2022120）
中国水产科学研究院基本科研业务费项目(2023TD81,20603022023018). （2023TD81,20603022023018）
