中国水产科学2024，Vol.31Issue(12)：1464-1473,10.DOI:10.12264/JFSC2024-0244
秦岭细鳞鲑视觉器官早期发育组织学观察
Histological observation of the visual organ of Brachymystax tsinlingensis Li 1966 at the early developmental stage
摘要
Abstract
Brachymystax tsinlingensis Li(1966)is sensitive to light and susceptible to death by strong light during long-term artificial breeding,especially during early developmental stages.The development of visual organs and the characteristics of retinal development were systematically observed using histological methods to analyze the physiological causes of this phenomenon and improve the efficiency of large-scale artificial breeding.The results showed that the embryo entered the organ differentiation stage,and the optic cup was formed 177 hours post-fertilization(HPF).At 213 HPF,a primitive retina and crystal sac were formed,and the cuboidal epithelium constituted the cornea.The crystal sac had developed into the lens,the cells of the neural layer of the retina had increased notably,and the staining of the pigment cell layer deepened at 357 HPF.At 452 HPF,the sclera appeared,and the retina differentiated into four layers(pigment epithelium,outer nuclear layer,inner nuclear layer,and optic ganglion cell layer).After 508 h,the fertilized egg broke the membrane and hatched larvae.The visual organs of hatchlings were relatively well-developed,and the lens is fully differentiated and has the same structure as that of the adult;the retina has been differentiated into 8 layers,and the pigment granules in the pigment layer are clearly visible;the sclera consists of elastic fibers and cartilage;the iris is in the form of a single-layer ring;and the choroid is present.At 5 days after hatching(DAH),a small number of cone cells appeared,and the visual cell layer was pure cone cell structure.At 10 DAH,the retina differentiated into a complete 10-layer structure,ON/SC was 1.27＞1,a few optic rods began to appear in the visual cell layer,and the inner nuclear layer was differentiated into three clear layers with the initial visual imaging system.At 12 DAH,ON/G was 1.07＞1,the retinal network was less convergent,and the number of nuclei in the outer nuclear layer was comparable to the number of ganglion cells,which was sufficient to receive signals from the visual cells.At 16 DAH,the mean value of the retinal bright-adapted pigmentation index(PI)was 0.75,whereas the mean value of the dark-adapted PI was significantly reduced to 0.55(P＜0.05).At 21 DAH,the number of optic rod cells increased,and the cornea differentiated into a complete 5-layer structure;at 41 DAH,the iris differentiated,the choroid was perfected,and all parts of the visual organs were fully developed.Therefore,the retinal structure and visual characteristics of B.tsinlingensis show strong light sensitivity,and vision plays a major role in its behavior and feeding activities.Reducing environmental light appropriately during early breeding of B.tsinlingensis could reduce the stress response and improve the survival rate of seedlings.The results of this study provide a reference for the proliferation,conservation,and utilization of B.tsinlingensis resources.关键词
秦岭细鳞鲑/视觉器官/视网膜/组织学/早期发育Key words
Brachymystax tsinlingensis/visual organ/retina/histology/early development分类
农业科技引用本文复制引用
张林,郭星辰,宋荣群,王桢璐,叶欢,熊冬梅,李志刚,邵俭..秦岭细鳞鲑视觉器官早期发育组织学观察[J].中国水产科学,2024,31(12):1464-1473,10.基金项目
国家自然科学基金项目(32002392) （32002392）
贵州省教育厅青年科技人才成长项目(黔教技[2024]37) （黔教技[2024]37）
陕西省重点研发计划项目(2018ZDXM-NY-037) （2018ZDXM-NY-037）
农业农村部淡水生物多样性保护重点实验室开放课题项目(LFBC1109). （LFBC1109）
