中国动物传染病学报2024，Vol.32Issue(6)：1-7,7.
LncRNA SNHG1调节鲍曼不动杆菌感染肺上皮细胞
Role Of LncRNA SNHG1 in Acinetobacter baumannii Infection of Lung Epithelial Cells
摘要
Abstract
Several studies have shown that aberrant expression of lncRNA SNHG1 is associated with inflammation-related diseases.To understand the role of SNHG1 in lung infection,Acinetobacter baumannii was used to infect human lung epithelial cells to establish a cellular infection model in this study.To further investigate the regulatory role of SNHG1 in the infection process,we constructed an overexpression vector of SNHG1 and synthesized its interfering RNA to investigate the effect of SNHG1 on the expression of antimicrobial peptide hBD-2 and cytokines IL-6 and IL-8.The results showed that the expression of hBD-2,IL-6 and IL-8 significantly increased after A549 cells were infected with A.baumannii,indicating that the infection model was successfully constructed.When SNHG1 was overexpressed in A549 cells,the transcript levels of IL-6 and IL-8 increased and the secretion of hBD-2 in the supernatant also increased.When SNHG1 was interfered,hBD-2 was slightly decreased.In conclusion,lncRNA SNHG1 was involved in the infection process through regulation of antimicrobial peptide hBD-2 and cytokines IL-6 and IL-8.关键词
鲍曼不动杆菌/肺上皮细胞/LncRNA-SNHG1/hBD-2/IL-6Key words
Acinetobacter baumannii/lung epithelial cells/LncRNA SNHG1/hBD-2/IL-6分类
医药卫生引用本文复制引用
高丽丽,秦廷洋,龙红,马晓春..LncRNA SNHG1调节鲍曼不动杆菌感染肺上皮细胞[J].中国动物传染病学报,2024,32(6):1-7,7.基金项目
青岛农业大学博士基金项目(663-1122005) （663-1122005）
贵州省科技厅基础研究计划(黔科合基础[2020]1Y350) （黔科合基础[2020]1Y350）
