中国动物传染病学报2024，Vol.32Issue(6)：42-49,8.
猪流行性腹泻病毒S蛋白不同截短体的免疫原性研究
Immunogenicity of Different Truncates of Porcine Epidemic Diarrhea Virus S Protein
摘要
Abstract
Porcine epidemic diarrhea(PED)is a highly contagious intestinal disease of piglets,which is caused by porcine epidemic diarrhea virus(PEDV).In this study,we used the HEK-293F cells to express different truncated proteins of the PEDV S gene(S,S1,and CTD).Four-week-old BALB/c mice were immunized with these proteins and serum samples were collected at 0,14 and 28 d post immunization.These three proteins were able to induce favorable immune responses in mice as confirmed by ELISA and virus neutralization.The proportion of CD3+CD4+CD8+lymphocyte subsets was determined in flow cytometry and IFN-γ in mouse cells and IL-4 were also examined to assess the immunogenicity of these truncated proteins.The results indicated that the PEDV S protein was the most immunogenic among these truncated proteins.This study provided helpful information for the development of a PEDV subunit vaccine.关键词
猪流行性腹泻病毒/S蛋白/真核表达/亚单位疫苗Key words
Porcine epidemic diarrhea virus/S protein/eukaryotic expression/subunit vaccine分类
农业科技引用本文复制引用
付琦媛,乔天一,郑宾宾,金宇航,季裕婷,杨雨杰,马增军,芮萍,宋涛..猪流行性腹泻病毒S蛋白不同截短体的免疫原性研究[J].中国动物传染病学报,2024,32(6):42-49,8.基金项目
河北省教育厅资助科研项目(QN2019097) （QN2019097）
国家自然科学基金青年科学基金(32102666) （32102666）
河北省重点研发计划项目(21324801D) （21324801D）
