中国动物传染病学报2024，Vol.32Issue(6)：75-80,6.
GPV、GoAstV和GPMV三重Nano-PCR检测方法的建立及应用
Development and Primary Application of a Nanoparticle-Assisted PCR Assay for Detection of GPV,GoAstV and GPMV
摘要
Abstract
In order to develop a simple,rapid and sensitive method for simultaneous detection of Goose parvovirus(GPV),Goose astrovirus(GoAstV)and Goose paramyxovirus(GPMV),specific primers were designed based on the conserved sequences of GPV,GoAstV and GPMV published on GenBank.Then,a nanometer PCR method was developed for detection of GPV,GoAstV and GPMV in combination with gold nanomaterials and optimized for the reaction conditions and clinic use.The results showed that the nano-PCR method developed here had good specificity as it had no amplification for Avian influenza virus(AIV),Fowl adenovirus(FAdV)and goose Tambusu virus(TMUV).The minimum detection limits of the nano-PCR method were 1.36×101 for AIV,1.36×103 for FAdV and 1.36×103 copies/μL for TMUV,which were 10 to 100 times higher than that of the conventional PCR.Subsequently,30 clinical goose samples were tested using this method,of which 1 sample was found GPV positive,2 samples GPMV positive,15 samples GoAstV positive and 1 sample for both GoAstV and GPV positive.These results showed that the nano-PCR method developed here was highly specific and sensitive and could be used for the clinical detection of GPV,GoAstV and GPMV.关键词
鹅细小病毒/鹅星状病毒/鹅副黏病毒/纳米PCRKey words
Goose parvovirus/Goose astrovirus/Goose paramyxovirus/nano-PCR分类
农业科技引用本文复制引用
苗艳,江波涛,金振华,田秋丰,于晨龙,张国华,罗天瑶,朱庆贺,陈亮,钟鹏,兰世捷,冯万宇,李丹,张蕾,沈思思..GPV、GoAstV和GPMV三重Nano-PCR检测方法的建立及应用[J].中国动物传染病学报,2024,32(6):75-80,6.基金项目
黑龙江省重点研发计划(GA21B004) （GA21B004）
齐齐哈尔市创新激励项目(CNYGG-2021005) （CNYGG-2021005）
