中国动物传染病学报2024，Vol.32Issue(6)：114-119,6.
牛呼吸道疾病多重荧光PCR(探针法)检测方法的建立及应用
Development and Application of TaqMan Multiplex Fluorescent PCR for Detection of Bovine Respiratory Diseases
摘要
Abstract
In this study,a triple fluorescence PCR was developed for the simultaneous detection of Infectious bovine rhinotracheitis virus(IBRV),Bovine viral diarrhea virus(BVDV)and Mycoplasma bovis.Specific primers and TaqMan probes were designed based on gB gene of IBRV,5'-UTR gene of BVDV and P48 gene of M.bovis for development of a multiple real-time PCR method followed by optimization for sensitivity,specificity and repeatability.The results showed that the method had high sensitivity with detection as few as 10 copy numbers,strong specificity without reaction with other bovine pathogens,good repeatability,low cost,and advantage over the national standard method in the testing clinical samples.Therefore,this multiple real-time PCR method developed here was suitable for testing a large number of clinical samples and performing epidemic monitoring,providing a scientific and reliable method for identifying pathogenic microorganisms of bovine respiratory diseases and reducing the economic loss of breeding.关键词
牛传染性鼻气管炎病毒/牛病毒性腹泻病毒/牛支原体/多重荧光PCRKey words
Bovine respiratory syndrome/Infectious bovine rhinotracheitis virus/Bovine viral diarrhea virus/Mycoplasma bovis/multiple real-time PCR分类
农业科技引用本文复制引用
陶攀,东笑,罗昌俊,张桂红,王贵平,贾爱卿..牛呼吸道疾病多重荧光PCR(探针法)检测方法的建立及应用[J].中国动物传染病学报,2024,32(6):114-119,6.基金项目
番禺区产业人才项目(2017-R02-4) （2017-R02-4）
