中国动物传染病学报2024，Vol.32Issue(6)：135-144,10.
禽腺病毒血清4型Fiber2 Head蛋白表达及其单克隆抗体筛选
Prokaryotic Expression of Fiber2 Head Protein of Fowl Adenovirus Serotype 4 and Preparation of Its Monoclonal Antibodies
摘要
Abstract
The purpose of this study was to prepare the specific monoclonal antibodies(mAbs)of fowl adenovirus serotype 4(FAdV4)Fiber2 Head protein.First of all,Fiber2 was truncated and expressed in prokaryotic soluble form.Then,BALB/c mice were immunized with the purified protein.After cell fusion,mAbs were screened by indirect ELISA and immunoperoxidase monolayer assay(IPMA)and determined for their reactivity,titers and subtypes.The results showed that Fiber2 Head protein was successfully expressed in prokaryotic system and had good biological activity.Eight monoclonal cell lines(5A5,1D5,7C9,4E5,3A11,5E11,9D7 and 6E3)were successfully obtained,which secreted mAbs that specifically recognized Fiber2 Head protein and FAdV4.In conclusion,mAbs that specifically reacted with Fiber2 Head protein and FAdV4 virus were successfully prepared in this study,which laid a foundation for the development of FAdV4 antigen and antibody detection reagents.关键词
禽腺病毒4型/Fiber2蛋白/单克隆抗体/免疫检测Key words
Fowl adenovirus serotype 4(FAdV-4)/Fiber2 protein/monoclonal antibody/immunoassay分类
农业科技引用本文复制引用
朱荣芳,季辉,吕凤霞,邢广旭,张改平,金前跃,柴永笑,陈晓,李伟,郭振华,卢清侠,柴书军,邢云瑞..禽腺病毒血清4型Fiber2 Head蛋白表达及其单克隆抗体筛选[J].中国动物传染病学报,2024,32(6):135-144,10.基金项目
河南省农业科学院自主创新项目(2022ZC67) （2022ZC67）
国家重点研发计划重点专项(2016YFD0500800) （2016YFD0500800）
河南省农业科学院杰出青年科技基金(2024JQ01) （2024JQ01）
