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2020-2023年贵阳市禽流感、新城疫的监测与分析OA北大核心CSTPCD

Surveillance and Analysis of Avian Influenza and Newcastle Disease in Guiyang from 2020 to 2023

中文摘要英文摘要

为了解贵阳市禽流感(AI)和新城疫(ND)疫病风险和免疫抗体水平情况,本研究于2020-2023年采集了119 779份禽血清样品和5158份禽拭子样品,采用血凝抑制试验对血清样品进行禽流感H5亚型、H7亚型和新城疫疫苗免疫抗体检测,利用荧光RT-PCR方法检测H5亚型AIV、H7亚型AIV和H9亚型AIV以及新城疫病毒,统计分析家禽禽流感和新城疫免疫抗体水平和感染情况.免疫抗体合格率统计结果显示,2020-2023年禽流感H5亚型免疫抗体样品合格率为90.75%~95.37%;禽流感H7亚型免疫抗体样品合格率为94.57%~96.57%;新城疫免疫抗体样品合格率为89.29%~94.14%.不同场点HPAIV和NDV免疫抗体样品合格率分别为83.65%~99.67%和85.83%~100%,商品化代养殖场和种禽场的免疫抗体样品合格率高于散养户.不同地区禽流感H5亚型免疫抗体样品合格率为82.46%~99.54%;禽流感H7亚型免疫抗体样品合格率为90.12%~99.77%;新城疫免疫抗体样品合格率为86.11%~100%.病毒阳性率的统计结果显示,2020-2023年间H5亚型AIV总阳性率为0.54%,H7亚型AIV总阳性率为0.08%,H9亚型AIV总阳性率为1.40%,未检测到新城疫病毒阳性样品.2020-2023年间H5亚型AIV、H7亚型AIV均只在市场环境中检出,总阳性率分别为1.34%和0.14%;H9亚型AIV在散养户、商品代养殖场和市场环境中均有检出,总阳性率分别为0.14%、0.62%和2.86%.2020-2023年间观山湖区、花溪区、南明区、乌当区、云岩区和息烽县等6个地区未检出H5亚型AIV、H7亚型AIV和H9亚型AIV;开阳县和清镇市仅检测到H9亚型AIV的存在,总阳性率分别为0.4%和0.7%;修文县H5亚型AIV、H7亚型AIV和H9亚型AIV总阳性率分别为2.99%、0.22%和4.42%;白云区H5亚型AIV、H7亚型AIV 和H9亚型AIV总阳性率分别为0.15%、0.15%和2.32%.监测结果表明,贵阳市新城疫防控措施效果较好,疫情发生风险低,禽流感疫苗免疫抗体总体水平整体较好;贵阳市HPAI疫情发生风险较低,个别地区发现H5亚型AIV和H7亚型AIV偶发,H9亚型AIV存在低水平程度的流行,尤其是市场环境中AIV污染情况较为严重.

In order to understand the risk of avian influenza and Newcastle disease and the level of immune antibodies in Guiyang City,119779 poultry serum samples and 5158 swab samples were collected from 2020 to 2023.The immune antibodies of avian influenza H5 subtype,H7 subtype and Newcastle disease vaccine were detected by hemagglutination inhibition test.The H5 subtype of Avian influenza virus,H7 subtype and H9 subtype of Avian influenza virus and Newcastle disease virus were detected by fluorescence RT-PCR.The level of immune antibodies and infection of avian influenza and Newcastle disease in poultry were statistically analyzed.The statistical results of the qualified rate of immune antibody showed that the qualified rate of immune antibody samples of avian influenza H5 subtype from 2020 to 2023 was 90.75%-95.37%.The qualified rate of avian influenza H7 subtype immune antibody samples was 94.57%-96.57%.The qualified rate of Newcastle disease immune antibody samples was 89.29%-94.14%.The qualified rates of highly pathogenic avian influenza virus(HPAIV)and Newcastle disease virus immune antibody samples in different sites were 83.65%-99.67%and 85.83%-100%,respectively.The qualified rates of immune antibody samples in commercial farms and breeding farms were higher than those in free-range farmers.The qualified rate of avian influenza H5 subtype immune antibody samples in different regions was 82.46%-99.54%.The qualified rate of avian influenza H7 subtype immune antibody samples was 90.12%-99.77%.The qualified rate of Newcastle disease immune antibody samples was 86.11%-100%.The statistical results of the positive rate of the virus showed that the total positive rate of H5 subtype AIV was 0.54%,the total positive rate of H7 subtype AIV was 0.08%,and the total positive rate of H9 subtype AIV was 1.40%.No positive samples of Newcastle disease virus were detected.From 2020 to 2023,H5 subtype AIV and H7 subtype AIV were only detected in the market environment,and the total positive rates were 1.34%and 0.14%,respectively.H9 subtype AIV was detected in free-range farmers,commercial farms and market environment,with the total positive rates of 0.14%,0.62%and 2.86%,respectively.From 2020 to 2023,H5 subtype of avian influenza virus,H7 subtype of avian influenza virus and H9 subtype of avian influenza virus were not detected in Guanshanhu District,Huaxi District,Nanming District,Wudang District,Yunyan District and Xifeng County.Only H9 subtype of avian influenza virus was detected in Kaiyang County and Qingzhen City,and the total positive rates were 0.4%and 0.7%,respectively.The total positive rates of H5 subtype of avian influenza virus,H7 subtype of avian influenza virus and H9 subtype of avian influenza virus in Xiuwen County were 2.99%,0.22%and 4.42%,respectively.The total positive rates of H5 subtype of avian influenza virus,H7 subtype of avian influenza virus and H9 subtype of avian influenza virus in Baiyun District were 0.15%,0.15%and 2.32%,respectively.The monitoring results showed that the effect of Newcastle disease prevention and control measures in Guiyang was good,the risk of epidemic was low,and the overall level of immune antibody of avian influenza vaccine was good.The risk of HPAI epidemic in Guiyang is low.H5 subtype AIV and H7 subtype AIV are occasionally found in some areas.H9 subtype AIV has a low level of prevalence,especially in the market environment.

杨峰;徐春志;蔺俐仲;杨源;张海;景书灏;袁翠霞;刘艳;刘亚娟;唐娇龙

贵阳市动物疫病预防控制中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081贵阳市乡村振兴服务中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081贵阳市乡村振兴服务中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081贵阳市动物疫病预防控制中心,贵阳 550081

畜牧业

禽流感新城疫血清学和病原学检测分析

Avian influenzaNewcastle diseaseserological and etiological detectionanalysis

《中国动物传染病学报》 2024 (6)

152-160,9

贵阳市人才创新创业资助项目(2015年第31号)

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