中国兽医科学2025，Vol.55Issue(1)：1-10,10.DOI:10.16656/j.issn.1673-4696.2025.0004
猪丁型冠状病毒非结构蛋白NSP9和NSP16抑制IFN-Ⅰ的表达
Inhibition of IFN-Ⅰ production by nonstructural proteins NSP9 and NSP16 of porcine deltacoronavirus
摘要
Abstract
This study was to investigate the regulatory effect of porcine deltacoronavirus(PDCoV)nonstructural proteins NSP9 and NSP16 in the regulation of interferon-Ⅰ(IFN-Ⅰ)in host cells.NSP9 and NSP16 were overexpressed in 293T cells.The effects of NSP9 and NSP16 on Sendai virus(SeV)-induced IFNβ were assessed by dual luciferase reporter system and qRT-PCR and the RIG-Ⅰ-like receptors(RLRs)signaling pathway were detected through qRT-PCR and Western-blot.The results showed that NSP9 and NSP16 significantly inhibited and decreased SeV-induced IFN-β promoter activity,transcriptional levels of IFN-β and interferon stimulated gene 15(ISG15).Overexpression of NSP9 significantly reduced the transcriptional levels of retinoic acid-inducible gene Ⅰ(RIG-Ⅰ),Melanoma differentiation associated gene 5(MDA5),Tank-binding kinase 1(TBK1)and interferon regulatory factor 3(IRF3)in RLRs,and significantly inhibited the expression of RIG-Ⅰ,MDA5,MAVS and TBK1.Overexpression of NSP16 reduces the transcriptional levels of RIG-Ⅰ and MDA5,and significantly inhibits RIG-Ⅰ expression and IRF3 phosphorylation.The results indicate that NSP9 and NSP16 of PDCoV may inhibit IFN-Ⅰ production by regulating RLRs,and provide a theoretical basis for elucidating the pathogenic mechanism of PDCoV,searching for new therapeutic targets,and developing new antiviral drugs.关键词
猪丁型冠状病毒/NSP9蛋白/NSP16蛋白/Ⅰ型干扰素Key words
porcine deltacoronavirus/NSP9 protein/NSP16 protein/type Ⅰ interferon分类
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刘思雨,许艺兰,李斌,程珂,秦毅斌,赵硕,欧阳康,何颖,卢冰霞,赵武,段群棚,许心婷,全琛宇..猪丁型冠状病毒非结构蛋白NSP9和NSP16抑制IFN-Ⅰ的表达[J].中国兽医科学,2025,55(1):1-10,10.基金项目
国家现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-2023-15-02) （nycytxgxcxtd-2023-15-02）
南宁市科技重大专项(20232039) （20232039）
广西基本科研业务费专项(桂科专项23-3/22-4/22-3/24-7/24-3/24-11) （桂科专项23-3/22-4/22-3/24-7/24-3/24-11）
广西兽医生物技术重点实验室开放基金项目(22-035-32-B-02,23-035-32-B-02,23-035-32-B-01) （22-035-32-B-02,23-035-32-B-02,23-035-32-B-01）
广西农业科技自筹经费项目(Z2023034,Z202225) （Z2023034,Z202225）
广西桂垦牧业科学研究与技术开发项目(桂垦牧科2022004) （桂垦牧科2022004）
