中国兽医科学2025，Vol.55Issue(1)：11-17,7.DOI:10.16656/j.issn.1673-4696.2024.0211
Tol-Pal系统tolQ基因对禽致病性大肠杆菌生物学特性及毒力的影响
Tol-Pal system tolQ gene affects the biological characteristics and virulence of avian pathogenic Escherichia coli
摘要
Abstract
This study focused on examining the role of the tolQ gene in the biological characteristics and pathogenicity of avian pathogenic Escherichia coli(APEC).Using the Red homologous recombination system and complementation plasmid,tolQ gene mutant and complemented strains of APEC were constructed.Then,we compared the the growth patterns,motility,morphology,biofilm formation capacity,survival in acidic and alkaline environments,cell adhesion and invasion capabilities,and pathogenicity of APCE50,△tolQ,and C△tolQ strains.The results indicated that the mutant strain exhibited decreased motility and altered bacterial morphology.The absence of the tolQ gene led to significant decreased biofilm formation capacity,reduced survival rate in acidic and alkaline conditions,and diminished serum bactericidal resistance.Cell and animal infection assays revealed that the tolQ mutant strain exhibited significantly diminished capacity to adhere to and invade cells,as well as attenuated virulence in animal.In summary,the tolQ gene plays a significant role in APEC motility,biofilm format ion,tolerance,and pathogenicity.These findings provide insights into the environmental adaptation,survival,and pathogenic mechanisms of APEC.The study contributes to our understanding of the environmental adaptability,adhesion and invasion abilities,and virulence mechanisms of APEC.关键词
禽致病性大肠杆菌/tolQ/生物学特性/毒力Key words
avian pathogenic Escherichia coli/tolQ/biological properties/virulence分类
畜牧业引用本文复制引用
李双阳,张贝贝,胡剑刚,王欣宇,祁晶晶,田明星,鲍衍清,石玉祥,王少辉..Tol-Pal系统tolQ基因对禽致病性大肠杆菌生物学特性及毒力的影响[J].中国兽医科学,2025,55(1):11-17,7.基金项目
国家自然科学基金项目(32172856) （32172856）
河北省三期现代农业产业技术体系蛋禽创新团队资助项目(HBCT2024260205) （HBCT2024260205）
上海市自然科学基金项目(22ZR1476100,23ZR1476600) （22ZR1476100,23ZR1476600）
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-SHVRI-07) （CAAS-ASTIP-2021-SHVRI-07）
