Tol-Pal系统tolQ基因对禽致病性大肠杆菌生物学特性及毒力的影响OA北大核心

This study focused on examining the role of the tolQ gene in the biological characteristics and pathogenicity of avian pathogenic Escherichia coli(APEC).Using the Red homologous recombination system and complementation plasmid,tolQ gene mutant and complemented strains of APEC were constructed.Then,we compared the the growth patterns,motility,morphology,biofilm formation capacity,survival in acidic and alkaline environments,cell adhesion and invasion capabilities,and pathogenicity of APCE50,△tolQ,and C△tolQ strains.The results indicated that the mutant strain exhibited decreased motility and altered bacterial morphology.The absence of the tolQ gene led to significant decreased biofilm formation capacity,reduced survival rate in acidic and alkaline conditions,and diminished serum bactericidal resistance.Cell and animal infection assays revealed that the tolQ mutant strain exhibited significantly diminished capacity to adhere to and invade cells,as well as attenuated virulence in animal.In summary,the tolQ gene plays a significant role in APEC motility,biofilm format ion,tolerance,and pathogenicity.These findings provide insights into the environmental adaptation,survival,and pathogenic mechanisms of APEC.The study contributes to our understanding of the environmental adaptability,adhesion and invasion abilities,and virulence mechanisms of APEC.