中国兽医科学2025，Vol.55Issue(1)：18-25,8.DOI:10.16656/j.issn.1673-4696.2025.0012
一起鹿呼吸道综合征相关病原的分析与鉴定
Analysis and identification of pathogens associated with deer respiratory syndrome
摘要
Abstract
In October 2023,a respiratory disease outbreak occurred among sika deer at a farm in Gansu Province.To investigate the etiology,nasal swabs and blood samples from infected deer and tissue samples from deceased animals were analyzed using qPCR and RT-qPCR.Eight pathogens associated with deer respiratory syndrome were detected,including Mycoplasma ovis,bovine parainfluenza virus type 3,bovine herpesvirus 1,bovine coronavirus,bovine respiratory syncytial virus,Mannheimia haemolytica,Pasteurella multocida,and Arcanobacterium pyogenes.The results showed that a total of four pathogens were detected,of which three were positive for M.ovis,four were positive for P.multocida,four were positive for M.haemolytica and three were positive for A.pyogenes,confirming that the respiratory disease on the farm was caused by a mixed infection of the above four pathogens.Isolation and identifi-cation of the pathogens resulted in the acquisition of one strain of M.haemolytica.Subsequent drug sensitivity testing showed that the strain was sensitive to cefepime,tobramycin,kanamycin,mide-camycin,and piperacillin,but resistant to ampicillin,cefazolin,cephalothin,cefuroxime,cefoxitin,cefoperazone.The study results offer valuable insights for the prevention,control,and targeted treatment of respiratory diseases in the deer farms.关键词
鹿呼吸道综合征/病原检测/混合感染/病原分离鉴定/药敏试验Key words
deer respiratory syndrome/pathogen detection/mixed infection/pathogen isolation and identification/drug sensitivity test分类
畜牧业引用本文复制引用
刘永杰,颜新敏,刘萍,张伟,兰喜,尚佑军,何继军,尚志锋,徐斌..一起鹿呼吸道综合征相关病原的分析与鉴定[J].中国兽医科学,2025,55(1):18-25,8.基金项目
"十四五"国家重点研发计划项目(2021YFD1800500) （2021YFD1800500）
国家现代农业产业技术体系专项(CARS-39-04B) （CARS-39-04B）
甘肃省科技特派团专项(22CX8NA012) （22CX8NA012）
