一起鹿呼吸道综合征相关病原的分析与鉴定OA北大核心

In October 2023,a respiratory disease outbreak occurred among sika deer at a farm in Gansu Province.To investigate the etiology,nasal swabs and blood samples from infected deer and tissue samples from deceased animals were analyzed using qPCR and RT-qPCR.Eight pathogens associated with deer respiratory syndrome were detected,including Mycoplasma ovis,bovine parainfluenza virus type 3,bovine herpesvirus 1,bovine coronavirus,bovine respiratory syncytial virus,Mannheimia haemolytica,Pasteurella multocida,and Arcanobacterium pyogenes.The results showed that a total of four pathogens were detected,of which three were positive for M.ovis,four were positive for P.multocida,four were positive for M.haemolytica and three were positive for A.pyogenes,confirming that the respiratory disease on the farm was caused by a mixed infection of the above four pathogens.Isolation and identifi-cation of the pathogens resulted in the acquisition of one strain of M.haemolytica.Subsequent drug sensitivity testing showed that the strain was sensitive to cefepime,tobramycin,kanamycin,mide-camycin,and piperacillin,but resistant to ampicillin,cefazolin,cephalothin,cefuroxime,cefoxitin,cefoperazone.The study results offer valuable insights for the prevention,control,and targeted treatment of respiratory diseases in the deer farms.