中国兽医科学2025，Vol.55Issue(1)：80-85,6.DOI:10.16656/j.issn.1673-4696.2025.0014
猪细小病毒TaqMan实时荧光定量PCR方法的建立与应用
Establishment and application of TaqMan real-time quantitative fluorescence method for porcine parvovirus
摘要
Abstract
In this study,a TaqMan real-time PCR method for PPV was established by designing the conserved sequence of the non-structural protein NS1 gene of porcine parvovirus(PPV)to achieve rapid detection of PPV.The results showed that the C,value of the detection method had a good linear rela-tionship with the standard plasmid at 1.0×1010 copies/µL-1.0×104 copies/μL,and the regression equation of the curve was y=-3.618 6x+42.01,R2=0.99,and the amplification efficiency was 189％.The reproducibility was good,and the coefficient of variation within and between groups was less than 1.089 8％.Good sensitivity,the lowest detection limit is 1 × 101 copies/µL.It has strong specificity and does not cross-react with porcine circovirus type 2,porcine reproductive and respiratory syndrome virus,porcine pseudorabies virus,porcine infectious gastroenteritis virus,African swine fever virus,and the clinical samples and virus copy number were detected by this method.The method estab-lished in this study has good reproducibility,high sensitivity and strong specificity,and is suitable for clinical detection and diagnosis of PPV.关键词
猪细小病毒/NS1蛋白/TaqMan实时荧光定量PCRKey words
porcine parvovirus/NS1 protein/TaqMan real-time PCR分类
农业科技引用本文复制引用
帅文娜,李兆龙,高飞,郭子强,李佳乐,罗梦,李丽薇,周艳君,姜一峰,童武,童光志..猪细小病毒TaqMan实时荧光定量PCR方法的建立与应用[J].中国兽医科学,2025,55(1):80-85,6.基金项目
国家重点研发计划资助项目(2022YFD1800800,2021YFD1801401) （2022YFD1800800,2021YFD1801401）
上海市自然科学基金资助项目(21ZR1476-900) （21ZR1476-900）
国家自然科学基金资助项目(32373050,32072861) （32373050,32072861）
中国农业科学院科技创新工程资助项目(CAAS-CS-LPDCP-202402) （CAAS-CS-LPDCP-202402）
上海市兽医生物技术重点实验室开放基金资助项目(BD1500010) （BD1500010）
