中国兽医科学2025，Vol.55Issue(1)：121-128,8.DOI:10.16656/j.issn.1673-4696.2025.0007
乐果对C2C12细胞内质网应激及成肌分化的影响
Effects of dimethoate on endoplasmic reticulum stress and myoblastic differentiation of C2C12 cells
摘要
Abstract
C2C12 cells were exposed to dimethoate(DIM)at various concentrations(0,0.3,0.6,0.9 mmol/L)for 24 h.Cell viability was determined by CCK-8,cell viability morphology and ultrastructure were observed through light microscopy and transmission electron microscopy,and the expression levels of Bip,PERK/eIF2α pathway-related proteins,MyOD,MyOG,and MyHC were detected by Western-blot.The re-sults indicated as follows:Compared with the 0 mmol/L group,the cell activity of the 0.3 mmol/L group was higher than that of the 0 mmol/L group.The expression levels of Bip and PERK/eIF2α pathway-related proteins.There were no significant differences in the expression levels of myodifferentiation-relat-ed proteins.The cell viability decreased significantly with the increase of DIM concentration(P＜0.01).The cell morphology was altered,the number of dead cells increased,and the cell ultrastructure was evidently damaged.The expression levels of Bip and PERK/eIF2α pathway-related proteins were sig-nificantly elevated(P＜0.05)or(P＜0.01).The expression levels of myodifferentiation-related pro-teins were significantly decreased(P＜0.05)or significantly declined(P＜0.01).PERK/eIF2α path-way-specific inhibitor GSK2606414 was added for DIM co-treatment,and the expression level of related proteins was reversed.The results demonstrated that DIM induced endoplasmic reticulum stress in C2C12 cells and activated the downstream PERK/eIF2α pathway,which mediated the impaired myoblast differen-tiation of C2C12 cells induced by DIM.关键词
乐果/C2C12/内质网应激/成肌分化Key words
dimethoate/C2C12/ER stress/myogenic differentiation分类
农业科技引用本文复制引用
康国静,陈嘉伟,邹辉,顾建红,袁燕,卞建春,刘学忠..乐果对C2C12细胞内质网应激及成肌分化的影响[J].中国兽医科学,2025,55(1):121-128,8.基金项目
江苏省农业科技自主创新资金项目[CX(18)3022] （18）
江苏省高校优势学科建设工程资助项目(PAPD) （PAPD）
