中国沼气2025，Vol.43Issue(1)：3-19,17.DOI:10.20022/j.cnki.1000-1166.2025010003
微生物角蛋白酶及其蛋白质工程研究进展
Research Progress on Microbial Keratinase and its Protein Engineering
摘要
Abstract
Keratinase is an important protease that can degrade complex keratin waste and enable the recycling of high-val-ue products such as polypeptides and amino acids.Currently,the activity and biological yield of natural keratinase by hos-ting organisms are relatively low,severely hindering the application of keratinase.The development of protein engineering provides an effective means to increase the production of keratinase and improve its functional properties.This article sys-tematically reviews the sources,physiological characteristics,and structural features of keratinase,summarizes the current methods of keratinase activity assay and the existing problems,and focuses on discussing the strategies and progress of ker-atinase protein engineering in the aspects of functional domain modification and multifunctional domain combination.Final-ly,we prospect the future application and directions of keratinase.关键词
角蛋白/角蛋白酶/酶活检测/蛋白质工程Key words
keratin/keratinase/enzyme activity assay/protein engineering分类
生物科学引用本文复制引用
张本旭,马诗淳,邓宇..微生物角蛋白酶及其蛋白质工程研究进展[J].中国沼气,2025,43(1):3-19,17.基金项目
海南省重点研发计划(ZDYF2021XDNY300) （ZDYF2021XDNY300）
河南省重点研发专项(241111321600) （241111321600）
中国农业科学院科技创新工程(CAAS-ASTIP-2016-BIOMA). （CAAS-ASTIP-2016-BIOMA）
