差异microRNA表达与女性髋部脆性骨折患者骨量丢失的相关性研究OA北大核心CSTPCD

Objective To explore relative factors for bone loss in postmenopausal female hip fracture patients through clinical statistics and bioinformatics methods.Methods The microRNA(miRNA)and messenger RNA(mRNA)expression profiles were sourced from the Gene Expression Omnibus(GEO).Target genes for the differential miRNA with the highest fold change were predicted by using TargetScan and miRDB tools.A total of 20 female patients with intertrochanteric femural fractures were included,and bone marrow was collected during surgery.The differential ex-pression of miRNA and target genes in bone marrow was verified.All data were tested for normal distribution.Linear corre-lation analysis was performed on each indicator between two variables,and the indicators related to bone density were in-cluded in the multiple linear regression equation,and the partial correlation analysis was performed between the final indi-cators and bone density.Results Totally 140 miRNAs were screened between high bone mass and low bone mass groups in the public database,with miR-595 having the highest|log2-FC|value.Finally,337 target genes were predicted,and CLDND1 was screened by intersecting with GSE100609.MiR-595 was upregulated and CLDND1 was downregulated in the high bone mass group of patients with hip fractures.Univariate correlation analysis showed a significant correlation be-tween age,weight,ferritin,miR-595,CLDND1,and bone density.Further multiple linear regression analysis showed a high correlation between age,miR-595,and bone density.After controlling for age variables,partial correlation analysis between miR-595 and bone density showed that they were significantly correlated.Conclusion miR-595 and its down-stream CLDND1 might be the independent relative factors for bone loss in postmenopausal women.