中国兽药杂志2025，Vol.59Issue(1)：29-35,7.DOI:10.11751/ISSN.1002-1280.2025.1.04
不同剂量注射用泮托拉唑钠在犬体内药代动力学研究
Pharmacokinetic Study of Different Doses of Pantoprazole Sodium Injections in Dogs
李冰 1李晶 2齐芸 1韩梦禹1
作者信息
- 1. 烟台赛普特检测服务有限公司,山东 烟台 264003
- 2. 山东爱士津生物技术有限公司,山东 烟台 264003
- 折叠
摘要
Abstract
In order to research the pharmacokinetics of pantoprazole sodium(Pan-Na)at different doses in dogs,an analytical method was established for the determination of pantoprazole sodium concentration in dog plasma by LC-MS/MS.Plasma samples were effectively extracted by acetonitrile while diclofenac sodium was used as internal standard.Separation was achieved on a Waters ACQUITY BEH C18(50 mm×2.1 mm,1.7 μm)column using acetonitrile-0.1％formic acid in water as the mobile phase.Under the electrospray positive ion mode,the multi-reaction monitoring mode was used for qualitative and quantitative analysis of pantoprazole.The plasma concentration of pantoprazole sodium in dogs was determined by this method after intravenous administration of three groups:low(2.00 mg/kg),medium(4.00 mg/kg)and high(8.00 mg/kg),and the main pharmacokinetic parameters were calculated by Phoenix WinNonlin software.The results indicated that pantoprazole sodium had a good linear relationship in the concentration range of 15.0～15000 ng/mL(R2≥0·999)and the lower limit of quantification was 15.0 ng/mL.The intra-and inter-batch precisions(RSD)were both less than 15.0％,the accuracy RE％was within 9.00％and the recovery was between 95.0％～101％The main pharmacokinetic parameters after intravenous injection of the three dose groups(2.00 mg/kg,4.00 mg/kg and 8·00 mg/kg)in beagle dogs were as follows:AUC0-t was 7714±2256 h·ng/mL,14389±5039 h·ng/mL and 41149±9193 h·ng/mL;AUC0-∞ was 7748±2248 h·ng/mL,14445±5042 h·ng/mL and 41250±9232 h·ng/mL;C0 was 8312±2460 ng/mL,16135±7401 ng/mL and 37969±16618 ng/mL;t1/2 was 0.638±0·0899 h,0.625±0·185 h and 0.783±0.151 h.The dose change had no significant effect on t1/2 and V(P>0·05).The results showed that the established method was simple,efficient and sensitive,the pantoprazole sodium had a faster metabolism and met the linear pharmacokinetic characteristics in dogs after intravenous injection of different dosages.关键词
泮托拉唑钠/超高效液相色谱-串联质谱联用/药动学/线性关系分析Key words
pantoprazole sodium/LC-MS/MS/pharmacokinetics/linear relationship analysis分类
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李冰,李晶,齐芸,韩梦禹..不同剂量注射用泮托拉唑钠在犬体内药代动力学研究[J].中国兽药杂志,2025,59(1):29-35,7.
