针灸推拿医学（英文版）2025，Vol.23Issue(1)：1-11,11.DOI:10.1007/s11726-024-1426-4
针刺对MPTP诱导的帕金森病模型小鼠SATB1/p21信号通路和衰老相关分泌表型的影响
Effects of acupuncture on SATB1/p21 signaling pathway and SASPs in MPTP-induced Parkinson disease model mice
摘要
Abstract
Objective:To observe the effects of acupuncture on the motor function of Parkinson disease(PD)model mice and to investigate the neuroprotective effects of acupuncture on PD from the perspective of cellular senescence. Methods:C57BL/6J mice were randomly divided into a normal control(NC)group,a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)group,an acupuncture(ACU)group,and a rasagiline(RAS)group,with 6 mice in each group.Except for the mice in the NC group,all mice were injected intraperitoneally with MPTP[30 mg/(kg·bw)]to establish a PD mouse model.After the models were successfully established,mice in the ACU group received acupuncture at Baihui(GV20)and bilateral Yanglingquan(GB34)for 15 min,once a day for 14 consecutive days.Mice in the RAS group were treated with gavage of rasagiline mesylate[0.5 mg/(kg·bw)],once daily for 14 d.Mouse balance and motor functions were detected using the mouse fatigue rotating rod apparatus.Immunohistochemistry staining was used to detect the number of tyrosine hydroxylase(TH)-positive neurons and the protein expression levels of special AT-rich sequence-binding protein 1(SATB1),p21,and p53 in the substantia nigra(SN)region of the mouse brain in each group.The glutathione peroxidase(GSH-Px)activity of mouse brain SN tissue was detected by enzyme-linked immunosorbent assay.The protein expression levels of interleukin(IL)-6 and senescence-associated β-galactosidase(SA-β-gal)in the SN tissue of mice in each group were detected by Western blotting.The relative expression of SATB1,p21,and p53 mRNA in the SN of each group was detected by real-time quantitative polymerase chain reaction. Results:Compared to the NC group,the overall rod performance(ORP)score,the number of TH-positive neurons,and GSH-Px activity in the SN region were significantly lower in the mice in the MPTP group(P<0.01);compared to the MPTP group,the ORP score,the number of TH-positive neurons,and GSH-Px activity were significantly increased in the ACU group and the RAS group(P<0.01 or P<0.05).Compared to the NC group,the protein levels of IL-6 and SA-β-gal in the SN tissue,the protein and mRNA expression levels of p21 and p53 were significantly increased(P<0.01);compared to the MPTP group,the protein levels of IL-6 and SA-β-gal in the SN tissue,the protein and mRNA expression levels of p21 and p53 were significantly decreased in the ACU group and the RAS group(P<0.01 or P<0.05).Compared to the NC group,the relative expression of SATB1 protein and mRNA in the SN of mice in the MPTP group was significantly decreased(P<0.01);compared to mice in the MPTP group,mice in the ACU group and the RAS group showed significant increases in the relative expression of SATB1 protein and mRNA(P<0.01 or P<0.05). Conclusion:Acupuncture can improve motor function and increase the number of TH-positive neurons in the SN of PD model mice.Its neuroprotective effect may relate to the regulation of the SATB1/p21 signaling pathway and the inhibition of cellular senescence-related biomarker expression in the SN.关键词
针刺疗法/穴,百会/穴,阳陵泉/帕金森病/信号传导/细胞衰老/小鼠Key words
Acupuncture Therapy/Point,Baihui(GV20)/Point,Yanglingquan(GB34)/Parkinson Disease/Signal Transduction/Cell Aging/Mice分类
医药卫生引用本文复制引用
李国娜,陈奕奕,程玲,乔宇,赵琛,王照钦,华雪桂,吴璐一,张惜音,沈琳,陈子怡,胡文清..针刺对MPTP诱导的帕金森病模型小鼠SATB1/p21信号通路和衰老相关分泌表型的影响[J].针灸推拿医学（英文版）,2025,23(1):1-11,11.基金项目
This work was supported by High Level Traditional Chinese Medicine Key Subjects of the State Administration of Traditional Chinese Medicine(国家中医药管理局高水平中医药重点学科建设项目,No.zyyzdxk-2023068) （国家中医药管理局高水平中医药重点学科建设项目,No.zyyzdxk-2023068）
Project of National Natural Science Foundation of China(国家自然科学基金项目,No.82205260) （国家自然科学基金项目,No.82205260）
Shanghai Pudong New Area Health System Famous Traditional Chinese Medicine Training Program(上海市浦东新区卫计委名中医培养计划,No.PWRzm2020-12) （上海市浦东新区卫计委名中医培养计划,No.PWRzm2020-12）
Discipline Construction of Pudong New Area Health Commission Acupuncture and Moxibustion Key Specialty of Traditional Chinese Medicine(浦东新区卫计委学科建设中医针灸重点专科,No.PWZzk2022-25). （浦东新区卫计委学科建设中医针灸重点专科,No.PWZzk2022-25）
