浙江中医药大学学报2025，Vol.49Issue(1)：1-8,8.DOI:10.16466/j.issn1005-5509.2025.01.001
运脾益肾通督方对强直性脊柱炎模型小鼠脊柱病变及免疫炎症的影响
Effect of Yunpi Yishen Tongdu Decoction on Spinal lesion and Immune Inflammation of Ankylosing Spondylitis Model Mice
摘要
Abstract
[Objective]To explore the alleviating effect of Yunpi Yishen Tongdu Decoction on spinal lesion and immune inflammation of ankylosing spondylitis(AS)model mice,and to provide a scientific basis for its clinical application.[Methods]A total of 42 female BALB/c mice were randomly divided into blank group,model group,western medicine group,low,medium and high dose Yunpi Yishen Tongdu Decoction groups.Except for blank group,the remaining 5 groups were injected with proteoglycans in the abdominal cavity to construct AS model.After modeling,the mice in blank group and model group were given 0.9％sodium chloride solution,the mice in western medicine group were given 3.57 mg·mL-1 celecoxib solution,and the mice in the low,medium,and high dose Yunpi Yishen Tongdu Decoction were given 0.9,1.8 and 3.6 mg·mL-1 of Yunpi Yishen Tongdu Decoction for 4 weeks,respectively.After gavage,the behavioral changes,serum inflammation cytokines,spinal lesions,and expression of receptor activator of nuclear factor-κB ligand(RANKL),receptor activator of nuclear factor-κB(RANK),and osteoclastogenesis inhibitory factor(OPG)in the spine were measured.[Results]Compared with blank group,model group had significantly increased total time in tail immobility,serum interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)level,spinal pathological score,RANKL and RANK expression(P＜0.01,P＜0.05),while the vertebral bone mineral density and OPG expression of the spine were significantly decreased(P＜0.01).After treatment with the Yunpi Yishen Tongdu Decoction,the latency time in tail immobility in AS mice was significantly reduced,along with decreased serum levels of IL-6 and TNF-α and a notable reduction in spinal pathological scores(P＜0.01,P＜0.05).Additionally,the treatment downregulated RANKL and RANK expression,upregulated OPG expression(P＜0.01,P＜0.05).[Conclusion]Yunpi Yishen Tongdu Decoction alleviates the bone destruction of AS by affecting the RANKL/RANK/OPG pathway.关键词
强直性脊柱炎/运脾益肾通督方/蛋白聚糖/RANKL/RANK/OPG通路/炎症/免疫性疾病/骨破坏/中药Key words
ankylosing spondylitis/Yunpi Yishen Tongdu Decoction/proteoglycan/RANKL/RANK/OPG pathway/inflammation/immune diseases/bone destruction/herbal medicine分类
医药卫生引用本文复制引用
邱富海,刘彬,邱一吾,汪曼莉,陈慧楠,何志兴,毛盈颖..运脾益肾通督方对强直性脊柱炎模型小鼠脊柱病变及免疫炎症的影响[J].浙江中医药大学学报,2025,49(1):1-8,8.基金项目
国家自然科学基金项目(81973663)National Natural Science Foundation Project(81973663) （81973663）
