植物保护2025，Vol.51Issue(1)：69-79,11.DOI:10.16688/j.zwbh.2024061
青海草原毛虫雌蛾性腺气味受体基因的鉴定及组织表达分析
Identification and tissue expression profiling of odorant receptor genes in sex pheromone glands of female Gynaephora qinghaiensis(Lepidoptera:Lymantriidae)
摘要
Abstract
To clarify the molecular characteristics and tissue-specific expression levels of odorant receptor(OR)genes in the sex pheromone glands of female Gynaephora qinghaiensis,this study employed bioinformatics and RT-qPCR techniques for gene identification and expression profile analysis.A total of 13 OR genes were screened and identified from the pheromone gland transcriptome database,including 12 GqinORs and 1 GqinORco.Only GqinOR1 had a signal peptide.Most GqinORs,except GqinOR3,GqinOR7 and GqinOR9,had one to three transmembrane domains,while GqinORco had eight transmembrane domains.The secondary structure of GqinORs is mainly composed of α helices.Phylogenetic analysis showed that GqinORs may share a common ancestor with ORs from Conogethes punctiferalis,and Streltzoviella insularis.Gene expression profile analysis showed that the expression levels of OR genes in different male and female tissues were significantly different(P＜0.05),with all 13 target genes expressed in sex pheromone glands,and GqinOR8 and GqinOR10 were highly expressed in the antennae and sex pheromone glands.This study provides an important basis for further research on olfactory functions in the odorant receptors of G.qinghaiensis.关键词
青海草原毛虫/气味受体/性腺/生物信息学分析/表达谱分析Key words
Gynaephora qinghaiensis/odorant receptors/sex pheromone glands/bioinformatics analysis/expression profile analysis分类
农业科技引用本文复制引用
刘占玲,高书晶,韩海斌,唐德靖,孔一森,潘学能,周渊涛..青海草原毛虫雌蛾性腺气味受体基因的鉴定及组织表达分析[J].植物保护,2025,51(1):69-79,11.基金项目
国家重点研发计划(2022YFD1401101) （2022YFD1401101）
