植物保护2025，Vol.51Issue(1)：141-148,8.DOI:10.16688/j.zwbh.2024037
云南芒果枯萎病病原菌鉴定
Identification of the pathogen causing mango wilt disease in Yunnan
摘要
Abstract
From 2016 to 2022,sixty suspected mango wilt disease samples were collected from the main mango cultivation base in Yongde county,Lincang city,Yunnan province.Using pathogen isolation,purification,morphological characterization,multi-gene phylogenetic analysis,and Koch's postulates verification,eight representative isolates(MG-1-1,MG-1-3,MG-1-7,MG-1-10,MG-2-1,MG-2-4,MG-2-8 and MG-2-10)were identified.All isolates emitted a fruity aroma on MYEA medium,formed prominent perithecia and ascospore masses,and displayed hat-shaped ascospores.Sequence homology analysis revealed 100％similarity in βT1,RPBH,MS204 and FG1093 gene sequences between isolates and Ceratocystis manginecans strains from Oman and Indonesia.A phylogenetic tree constructed from these gene sequences grouped the eight isolates with C.manginecans with a bootstrap support of 93 and a Bayesian support value of 0.98.Pathogenicity tests on mango seedlings using isolates MG-1-10 and MG-2-8 revealed that they caused symptoms consistent with those observed in the field.This study thus confirms C.manginecans as the pathogen responsible for mango wilt disease in Yunnan.关键词
芒果/枯萎病/病原鉴定/Ceratocystis manginecansKey words
mango/wilt disease/pathogen identification/Ceratocystis manginecans分类
农业科技引用本文复制引用
张治萍,张竹青,窦敏,范福园,任云贵,刘波,沙素飞,李健强..云南芒果枯萎病病原菌鉴定[J].植物保护,2025,51(1):141-148,8.基金项目
国家自然科学基金(31571958) （31571958）
云南省李健强专家工作站(202105AF150046) （202105AF150046）
云南省"兴滇英才支持计划"青年人才(XDYC-QNRC-2022-0710) （XDYC-QNRC-2022-0710）
云南农业大学科研启动基金(KY2022-64) （KY2022-64）
