中医药信息2025，Vol.42Issue(2)：17-20,4.DOI:10.19656/j.cnki.1002-2406.20250203
怀牛膝-何首乌药对治疗小鼠骨质疏松的最佳配比研究
Optimal Ratio Study of Achyranthes Radix et Rhizoma and Polygoni Multiflori Radix Couplet Medicinals for Treating Osteoporosis in Mice
摘要
Abstract
Objective:To investigate the therapeutic effects of different ratios of the Achyranthes Radix et Rhizoma(Huai Niu Xi)and Polygoni Multiflori Radix(He Shou Wu)couplet medicinals on osteoporotic mice and to determine the optimal ratio for anti-osteoporosis efficacy.Methods:A total of 130 KM mice(half male and half female)were randomly divided into a model group,blank group,positive drug group,and treatment groups with different ratios of Achyranthes Radix et Rhizoma and Polygoni Multiflori Radix(1∶2,2∶1,3∶2,2∶3,1∶1,4∶3,3∶4,5∶2,0∶1,1∶0),with 10 mice in each group.The model,positive drug,and treatment groups were administered retinoic acid by gavage to induce osteoporosis,while the blank group received saline.The herbal couplet medicinalss were prepared through water reflux and administered by gavage,with alendronate sodium as the positive drug.Treatment began 12 hours post-modeling and continued for 15 days.Blood was collected 24 hours after the final administration,and ELISA was used to measure RANKL,OPG,PTH,TRACP-5b,and BALP levels,as well as calculate the OPG/RANKL ratio.Results:Compared to the blank group,BALP,OPG,and OPG/RANKL were significantly reduced in the model group(P<0.01,P<0.05),while TRACP-5b,RANKL,and PTH levels were significantly elevated(P<0.05,P<0.01),confirming the successful induction of osteoporosis in the model group.Different treatment groups impacted TRACP-5b,PTH,BALP,OPG,RANKL,and OPG/RANKL levels,indicating that the Achyranthes Radix et Rhizoma and Polygoni Multiflori Radix couplet medicinals promotes bone formation and inhibits bone resorption.Comprehensive scoring results were 4.98,2.77,2.89,2.13,2.65,2.53,3.11,2.60,2.14,and 1.63 for the ratios 1∶2,2∶1,3∶2,2∶3,1∶1,4∶3,3∶4,5∶2,0∶1,and 1∶0,respectively,with the 1∶2 ratio showing the highest anti-osteoporosis efficacy.Conclusion:Both Achyranthes Radix et Rhizoma and Polygoni Multiflori Radix alone and in combination have a therapeutic effect on osteoporosis,but the combination is more effective,with the 1∶2 ratio yielding the best results.关键词
怀牛膝-何首乌/骨质疏松/维甲酸/配比/ELISAKey words
Achyranthes Radix et Rhizoma-Polygoni Multiflori Radix/Osteoporosis/Retinoic acid/Ratio/ELISA引用本文复制引用
石舒骐,石安骐,林磊婷,王泽,苏羽,孙艳涛,王冰..怀牛膝-何首乌药对治疗小鼠骨质疏松的最佳配比研究[J].中医药信息,2025,42(2):17-20,4.基金项目
辽宁中医药大学"大学生创新创业训练计划资助"项目(X202310162048) （X202310162048）
