张琼月 1张梦洁 1闫李婕 1盛艺菲 1韩雨萌 1李燕1
作者信息
- 1. 北京中医药大学东直门医院,北京 100700
- 折叠
摘要
Abstract
Objective:To analyze the clinical efficacy of Xinyihua powder in the treatment of children with allergic rhinitis(AR)of lung meridian with latent heat syndrome,and to explore the mechanism of Xinyihua powder in the treatment of children with AR based on network pharmacology and molecular docking technology.Methods:In terms of clinical research,thirty-five children with AR of lung meridian with latent heat syndrome were treated with Xinyihua powder,the improvement of clinical symptoms and signs was observed after two weeks of treatment.In terms of mechanism,the Traditional Chinese Medicine Systemic Pharmacology Database and Analysis Platform(TCMSP)was used to screen active components and potential targets of the core traditional Chinese medicines in Xinyihua powder,and CytoScape 3.10.1 software was employed to construct the"traditional Chinese medicine-active components-targets"network diagram.DrugBank Online,On-line Mendelian Inheritance in Man(OMIM),Therapeutic Target Database(TTD),and GeneCards databases were retrieved to obtained AR-related targets.R language 4.3.2 was used to draw Venn diagram,the intersection targets were firstly imported into the STRING database,and then imported into CytoScape 3.10.1 software to construct protein-protein interaction(PPI)network diagram,and the key targets were screened.Key targets were imported into the DAVID database for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Molecular docking of key traditional Chinese medicine compounds with key target proteins was performed by using AutoDock 4.2 software,with results visualized through PyMol 2.4.0.Results:In terms of clinical research,the total effective rate after treatment was 91.43％,with 21 cases showing marked improvement,11 cases showing improvement and 3 cases showing no effect.Compared with before treatment,the scores of major symptoms,minor symptoms and signs decreased after treatment(P＜0.05).In terms of mechanism,five key active components(quercetin,kaempferol,β-sitosterol,stigmasterol and luteolin)and 243 potential targets were identified from the core traditional Chinese medicine of Xinyihua powder.Totally 2,426 AR-related targets were identified,with 124 intersection targets between Xinyihua powder and AR.Five key targets were identified:tumor necrosis factor(TNF),interleukin-6(IL-6),RAC-α serine/threonine protein kinase(AKT1),IL-1β and prostaglandin endoperoxidase-2(PTGS2).GO enrichment analysis indicated that these key targets were mainly involved in inflammatory responses and estrogen response element binding.KEGG enrichment analysis revealed the involvement in pathways such as the IL-17 signaling pathway,T helper cells(Th17)differentiation pathway and TNF signaling pathway.Molecular docking demonstrated that the binding energies of 25 docking pairs were ≤-5.00 kcal/mol.Conclusion:The clinical efficacy of Xinyihua powder in the treatment of children with AR is significant,mainly through the multi-component,multi-target and multi-pathway.关键词
儿童/过敏性鼻炎/辛夷花散/临床研究/网络药理学/分子对接Key words
children/allergic rhinitis/Xinyihua powder/clinical research/network pharmacology/molecular docking分类
医药卫生
